Polioviruses can cause polio, also known as poliomyelitis. The virus has three serotypes. The laboratory diagnostic methods for poliovirus include isolation and identification of viruses, molecular biological methods, and serological diagnostic methods. Although the isolation and identification of viruses is a common method, it is difficult to carry out in general clinical laboratories. Molecular biology methods are mainly PCR and nucleic acid hybridization. Serological diagnostic methods include neutralization test, indirect immunofluorescence test, reverse indirect hemagglutination test and ELISA method.Basic Information
Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection
Applicable gender: whether men and women apply fasting: not fastingAnalysis results:
Positive indicates polio.
The normal value is negative.Clinical significance
After being infected with poliovirus, the clinical manifestations may be mild or paralyzed. Positive poliovirus antibodies can be used to diagnose polio.
People who need to be tested:
Nerve paralysis, muscle paralysis, etc.Positive results may be diseases: polio, polio sequelae precautions
Before the examination: It is necessary to check the symptoms of viral blood, nerve paralysis, muscle paralysis, respiratory paralysis and so on.
After the examination: After the body is infected with poliovirus for several days, the corresponding antibodies are produced in the body.
Unsuitable for people: those who do not have an indication for examination should not do this check.Inspection process
1. Cultivate bacteria in large plants.
2. Removal of virus-rich medium
3. Concentrate the viral material by precipitation.
4. Final purification of the virus by density gradient centrifugation.Not suitable for the crowd
Those who do not have an indication for examination should not do this check.Adverse reactions and risks
Generally no complications and harm.