Sister chromatid exchange
Sister chromatid exchange is a new approach to cytogenetic research in recent years. The principle is that when cells are exposed to 5-bromodeoxyuridine (Brdu), Brdu can be used as a nucleotide precursor to specifically incorporate thymidine into the newly synthesized DNA strand. As long as the two cell replication cycles are passed, one strand of the DNA in one of the sister chromatids can be incorporated into Brdu, while the other strand is doubled in the DNA duplex. The cells doped with Brdu. After differentiation and staining, there are obvious differences between the two sister chromatids of the same chromosome, one monomer is dark and the other is light.Basic Information
Specialist classification: growth and development check classification: genetic testing (DNA)
Applicable gender: whether men and women apply fasting: not fastingAnalysis results:
SCE is elevated.
Abnormal results may cause an increase in SCE (compared to normal controls) when exposed to certain drugs or chemicals that have a mutating effect, as well as certain viral infections and short-wave radiation.
SCE is elevated.Clinical significance
Exposure to or taking of certain mutagenic drugs or chemicals, as well as certain viral infections and short-wave radiation, can cause an increase in SCE (compared to normal controls). SCE is a useful indicator and superior to the observation of chromosomal aberrations when studying the possibility of a new drug.
People who need to be tested:
It is necessary to check whether the population has recently been exposed to or taken certain drugs that have mutagenic effects, or to check for chromosomal diseases.Positive results may be diseases: children's facial erythema dwarf syndrome precautions
There are no special precautions for doing chromosome examinations, you can do it at any time.Inspection process
After the blood was drawn, the chromosomes of the human cells were examined by electron microscopy.Not suitable for the crowd
Taboo people: No.Adverse reactions and risks
It does not generally cause special complications and hazards.