Anti-hepatitis A virus IgA antibody
After the hepatitis A virus is infected into the human body, three kinds of antibodies against HAV-IgM, anti-HAV-IgA, and anti-HAV-IgG may be present. Among them, anti-HAV-IgM and anti-HAV-IgA appeared earlier, anti-HAV-IgG appeared later, and clinical detection of anti-HAV-IgM is the basis for early infection of hepatitis A virus.Basic Information
Specialist classification: Infectious disease inspection and classification: biochemical examination
Applicable gender: whether men and women apply fasting: fastingAnalysis results:
Positive in hepatitis A. Clinical diagnosis of hepatitis A is based on anti-HAV-IgM-positive and alanine aminotransferase (ALT) elevation.
Normal value: negative.Clinical significance
Positive in hepatitis A. Clinical diagnosis of hepatitis A is based on anti-HAV-IgM-positive and alanine aminotransferase (ALT) elevation.Positive results may be diseases: hepatitis A precautions
Anti-HAV-IgG appears later and lasts longer and is often negative at the beginning of the infection. It is commonly used in epidemiological investigations.Inspection process
1. Laboratory materials: blood.
2. Determination principle of anti-hepatitis A virus IgA antibody: capture specific IgA in the serum to be tested with anti-human μ chain, then react with HAV and specific IgA antibody, add HAV antibody, and finally add substrate color. .
3. Reagents: There are a complete set of kits available in China. Reagents that have been certified by the China National Institute for the Control of Pharmaceutical and Biological Products and have a “certified” anti-counterfeit label must be used as required. Including: HAV antigen, anti-human μ chain antibody, and the like.
4. Method of operation: Dilute the sample to be tested with 1:500, add 50 μl per well (with negative and positive control at the same time), add 50 μl of accelerator, shake rapidly for 1 min, set at 37 ° C for 20 min. Discard the liquid in the well of the reaction plate and air dry. Wash 4 times with washing solution and air dry. 50 μl of hepatitis A antigen was added to each well, and 50 μl of the enzyme-labeled antibody mixture was added, and the mixture was rapidly shaken for 1 min at 37 ° C for 30 min. Wash 4 times with washing solution and buckle dry. 100 μl of the substrate solution was added to each well and placed at 37 ° C for 10 min. 50 μl of stop solution was added to each well.Not suitable for the crowd
Those who do not have an indication for examination should not do this check.Adverse reactions and risks
Generally no complications and harm.