Anti-hepatitis E virus IgG antibody
HEV is the causative agent of hepatitis E and belongs to the RNA virus. The symptoms caused by HEV infection are similar to those of hepatitis A, which may cause epidemics, and water contaminated by HEV is transmitted through the feces-mouth. Serological tests are generally performed by ELISA to detect anti-HEV-IgG.Basic Information
Specialist classification: Infectious disease examination and classification: liver function examination
Applicable gender: whether men and women apply fasting: fastingAnalysis results:
Anti-HEV-IgG positive and anti-HEV-IgM negative suggest a previous infection.
Anti-HEV assays are commonly used in the diagnosis of HEV infection. Anti-HEV-IGM positive is a sign of acute infection, but it is easy to miss because of disappearance. Anti-HEV-IgG once positive can not make a diagnosis of recent HEV infection, where the hepatitis E recovery period anti-HEV-IgG titer ≥ acute phase 4 times, suggesting that HEV is newly infected, has diagnostic significance. Simultaneous determination of anti-HEV-IgG and anti-HEV-IgM contributes to clinical analysis. Anti-HEV-IgM positive is helpful for the diagnosis of acute hepatitis E, anti-HEV-IgG positive and anti-HEV-IgM negative suggesting previous infection.Positive result may be disease: pre- treatment of hepatitis E virus
As to whether anti-HEV is a protective antibody, it tends to have some protection at present, but it is still not clear.Inspection process
1. Principle of determination of anti-hepatitis E virus IgG antibody
The HEV-specific polypeptide expressed by artificial synthesis or genetic engineering is coated with a microplate, and the serum to be tested is added to react with it, and the anti-human IgG is added and the substrate is colored.
Use a commercial kit officially approved by the health department. (The antigen is 2 to 3 polypeptide fragments, and the coating solution is preferably 10 mmol/L pH 7.4 PBS. The sample dilution is pH 7.2 Tris-HCl, 10 g/LBSA, 1 ml/L polysorbate 20. Enzyme-labeled anti-human IgG.)
3, the operation method
Operate according to the kit instructions, or as follows: The antigen polypeptide is coated on a microplate, washed at room temperature overnight, 3 times. Add 100 μl of the diluted solution to each well, and make 5 μl of the sample. Set a negative and positive control at the same time, wash at 37 ° C for 30 min, and wash 6 times. 100 μl of HRP anti-human IgG was added to each well, and washed at 37 ° C for 30 min for 6 times. 100 μl of substrate solution (TMB-H2O2) per well was placed at room temperature for 15 min. The reaction was stopped by adding 10 g/LSDS 50 μl, and the 630 nm A value was measured.Not suitable for the crowd
Those who do not have an indication for examination should not do this check.Adverse reactions and risks
Generally no complications and harm.