Coxsackie virus antibody
Coxsackievirus belongs to the genus of the small RNA virus family Enterovirus, and the genome is single-stranded RNA. Cox is divided into two groups, A and B. Group A is divided into 24 (1 to 24) serotypes, and group B is divided into 6 (1 to 6) serotypes. It can detect Cox-IgM and Cox-IgG antibodies, combined with Cox-RNA detection, which is helpful for the diagnosis of this disease.Basic Information
Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection
Applicable gender: whether men and women apply fasting: fastingAnalysis results:
Normal, not infected with Coxsackie virus.
Prompt Coxsackie virus infection, common in type 1 diabetes, diabetes, Coxsackie virus infection, diarrhea, viral myocarditis, myocarditis and other diseases.
(1) Coxsackie virus antibodies can be detected in serum of patients with type 1 diabetes.
(2) Coxsackie virus antibody positive can also be seen in aseptic encephalitis caused by Coxsackie virus infection, pediatric pneumonia, diarrhea in children, muscle weakness, viral myocarditis and early abortion in pregnant women.Positive results may be diseases: Coxsackie virus infection, neonatal coxsackievirus B infection, Coxsackie virus rash, pediatric eccle and Coxsackie virus infection, type I diabetes precautions
Inappropriate people: generally no special population.
Taboo before the examination: It is necessary to cooperate with the doctor to write the correct name, neat and tidy, to avoid confusion caused by the same name or similar names. With these in mind, blood draws are more convenient and faster, and you can better save yourself time for diagnosis.
Requirements for inspection: Do not wear clothes that are too small or too tight in cuffs to avoid the sleeves being too tight when blood is drawn or the sleeves are too tight after blood drawing, causing blood vessels in the arms. Different laboratory items should be asked by the doctor and treated differently.Inspection process
1, laboratory materials
2, the principle of Coxsackie virus antibody determination
The specific antiviral immune serum (neutralizing antibody) binds to the virus, so that the virus can not be adsorbed to the sensitive cells, or the binding inhibits its adsorption and shelling, and thus the virus loses its ability to infect. This reaction is not only qualitative, but also in terms of quantity. That is, the infectivity of neutralizing a certain amount of virus must have a certain titer of immune serum.
The neutralization test is based on the determination of the infectivity of the virus, so the test must be carried out in vivo in animals, tissue culture cells, etc., and virus-sensitive cells, animals or chicken embryos must be used as materials. The determination of neutralizing antibody titration is based on the comparison of the residual infectivity of the virus after neutralization by immune serum, so the control experiment is very important.
Test tubes, pipettes, virus dilutions, incubators, Hep-2 or RD or other sensitive cells, known strains and titers of Coxsackie virus standard strains.
4, the operation method
(1) The serum to be tested was inactivated at 56 ° C for 30 min and then serially diluted.
(2) Each dilution of serum was mixed with an equivalent standard strain (0.1 ml containing 100 TCID50) and placed at 37 ° C for 2 h.
(3) The serum-virus mixture was inoculated into cells, and four tubes were inoculated at each dilution, 0.2 ml per tube. At the same time, virus and cell control are set.
(4) Culture at 37 ° C, observe every other day, and judge the result in 7 days.
A neutral method can also be used for the neutralization test. In addition to tissue culture neutralization assays, animal neutralization assays can also be used.
(5) The micro-method reference poliovirus is carried out.Not suitable for the crowd
Those who do not have an indication for examination should not do this check.Adverse reactions and risks
1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding.
2. Risk of infection: If you use an unclean needle, you may be at risk of infection.