Saliva is a fluid secreted by many oral glands. The composition of saliva is very complicated, and some are closely related to plasma such as uric acid, urea, etc., but some components are not affected by plasma concentrations such as protein, electrolyte bicarbonate and the like. Bicarbonate also plays an important role in physiology. The blood contains a buffer solution composed of H2CO3-HCO3- to resist large pH changes and maintain a suitable pH for biomolecules such as enzymes.Basic Information
Specialist classification: oral examination classification: body fluid examination
Applicable gender: whether men and women apply fasting: not fastingAnalysis results:
Lowering can be seen in pancreatitis.
Saliva bicarbonate: 21-50mmol/L
21 to 50 mmol/L.Clinical significance
Lowering can be seen in pancreatitis.Low results may be diseases: chronic pancreatitis, pancreatitis precautions
1, 10mmol / L NaOH can easily absorb CO2 in the air, so that the alkalinity is reduced, so it is necessary to pack and store, and mix reagents to make the end point obvious.
2, saliva should be fresh, the concentration of acid and alkali solution should be accurate.Inspection process
The specimen is sent immediately after the specimen is collected. The test operation is as follows:
1. Take 2 large test tubes, each adding 0.1ml of phenol red indicator and 0.1ml of saliva (after dilution).
2, 1 tube plus 2.5ml of normal saline as a control tube, the other tube is the measuring tube, accurately add 10mmol / L HCl 0.5ml, shake for 1min, make CO2 escape, add 2.0ml of normal saline, drip with 10mmol / L NaOH The same color as the control tube is the end point. The number of ml of 10 mmol/L NaOH used for titration was recorded.Not suitable for the crowd
no.Adverse reactions and risks