Gastric juice -glucuronidase
--glucuronidase (β-G) is a matrix degrading enzyme involved in tumor invasion and metastasis. β-G is a medium-acid lysosomal enzyme that hydrolyzes the main component of the basement membrane, proteoglycan, and thus participates in tumor invasion and metastasis.Basic Information
Specialist classification: Digestive examination classification: biochemical examination
Applicable gender: whether men and women apply fasting: not fastingAnalysis results:
Negative: 3.48 ~ 5.72U.
Positive (≥10U): gastric cancer (83.3%), atrophic gastritis with intestinal metaplasia (21.9%).
3.48 ~ 5.72U.Clinical significance
Positive (≥10U) gastric cancer (83.3%), atrophic gastritis with intestinal metaplasia (21.9%).Positive results may be diseases: gastric cancer, atrophic gastritis precautions
The β-Ga enzyme is widely distributed, and the enzyme activity in the liver is extremely high. There are three isozymes. The recent pH values are: 3.8, 4.5 and 5.2, respectively.Inspection process
ELISA can be used to determine antigens and can also be used to determine antibodies. Three essential reagents in this assay: 1 solid phase antigen or antibody; 2 enzyme labeled antigen or antibody; 3 substrate for enzyme reaction. Depending on the source of the reagent and the nature of the specimen and the specific conditions of the assay, various types of assay methods can be devised.
Use ELISA method:
The principle of ELISA is based on the immobilization of antigens or antibodies and the enzymatic labeling of antigens or antibodies. The antigen or antibody bound to the surface of the solid support maintains its immunological activity while retaining the activity of the enzyme. At the time of the assay, the test specimen (the antibody or antigen to be assayed therein) and the enzyme-labeled antigen or antibody are reacted in different steps with the antigen or antibody on the surface of the solid phase carrier. The antigen-antibody complex formed on the solid phase carrier is separated from other substances by a washing method, and finally the amount of the enzyme bound to the solid phase carrier is proportional to the amount of the test substance in the sample. After the substrate of the enzyme reaction is added, the substrate is catalyzed by the enzyme to become a colored product, and the amount of the product is directly related to the amount of the test substance in the sample, so that qualitative or quantitative analysis can be performed according to the depth of the color. Since the catalytic efficiency of the enzyme is high, the result of the reaction can be greatly amplified, so that the measurement method achieves high sensitivity.Not suitable for the crowd
Gastric perforation is prohibited in patients with gastric perforation.Adverse reactions and risks
Generally no complications.