Bacterial CAMP test
The CAMP test of bacteria is based on the ability of group B streptococci to produce CAMP factor, which promotes the activity of staphylococcal B-hemolysin to dissolve red blood cells, so the hemolysis force increases at the junction of two bacteria (group B streptococci and staphylococcus). A test performed on the principle of a sagittal (half-moon) hemolysis zone. On the blood plate, ATCC25923 was inoculated as a horizontal line, and a line was inoculated with the test bacteria in the vertical direction. The 2 lines could not intersect and the distance was 3 to 4 mm. The results were observed after incubation at 35 ° C for 18-24 hours.Basic Information
Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection
Applicable gender: whether men and women apply fasting: not fastingAnalysis results:
Streptococcus agalactiae, also known as Group B Streptococcus, is a beta-hemolytic streptococcus that is found in the human gastrointestinal tract. About a quarter of healthy adult women carry group B streptococci in the reproductive tract, and most carriers are asymptomatic. Group B streptococcus mainly infects newborns through vertical transmission during birth or horizontal transmission after delivery.
Early onset is mostly caused by bacterial infections in the birth canal. The onset of the disease manifested as pulmonary infection, sepsis and meningitis within 7 days after birth. Late onset is mostly caused by maternal postpartum complications. Older people, especially those with certain chronic diseases, can be infected regardless of gender.
Negative: Streptococcus agalactiae, also known as Group B Streptococcus, is a beta-hemolytic streptococcus that is present in the human gastrointestinal tract. About a quarter of healthy adult women carry group B streptococci in the reproductive tract, and most carriers are asymptomatic. Group B streptococcus mainly infects newborns through vertical transmission during birth or horizontal transmission after delivery.Clinical significance
Mainly used for the identification of group B streptococcus (positive), other streptococci are negative.
1, early onset: because the baby is caused by bacterial infection in the birth canal. The onset of the disease manifested as pulmonary infection, sepsis and meningitis within 7 days after birth.
2, late onset: mostly caused by maternal postpartum complications. It occurs within 7 to 30 days of birth. Septicemia and meningitis are more common types of type III, especially meningitis, more than 90% are caused by type III. Young women are prone to urinary tract infection and endometritis of B-streptococcus after gynaecological examination and pregnancy delivery. Pneumonia, meningitis, liver abscess and sepsis can also occur.
3, older, especially those who have some chronic diseases, both men and women can be infected.Positive results may be diseases: meningitis, neonatal sepsis, negative results of sepsis may be diseases: Helicobacter pylori infection precautions
Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene.
Requirements for inspection: Actively cooperate with the doctor.Inspection process
The CAMP experiment assisted in the initial identification of Group B Streptococcus.
Method: On the sheep blood or horse blood agar plate, the β-hemolytic Staphylococcus aureus was first inoculated with a horizontal line. Then, the test bacteria in the culture solution taken out from the site to be inspected are inoculated vertically with the previous line, and the two should be separated by 1 cm, and incubated at 35 ° C for 18 to 24 hours, and the results are observed. A positive positive control should be performed for each trial.
RESULTS: The arrow-type hemolysis zone was positive at the junction of the two bacterial lines.Not suitable for the crowd
People who are asymptomatic and have no indications for examination do not need to be tested.Adverse reactions and risks
Generally no harm.