HPx is a multi-molecular form of protein synthesized by hepatocytes. It has a molecular weight of 57,000 and has high affinity for hemoglobin and hemin. 1 mg of HPx can be combined with heme or hemin in a ratio of 1.11 g to form a complex. The liver is cleared. Heme-binding protein (hemexin, Hpx) has a molecular weight of 57,000, a single-chain polypeptide with a sugar content of about 22%. The content in normal plasma is 500-1000 μg/L, and it has specific binding ability to free heme. It can be combined with globin to treat hemoglobin. When extensive hemolysis, plasma-bound globin is depleted, and free hemoglobin in the circulation can be degraded into two parts, globin and heme.

Basic Information

Specialist classification: cardiovascular examination classification: biochemical examination

Applicable gender: whether men and women apply fasting: fasting

Analysis results:

Below normal:
Decreased serum levels are seen in severe hemolytic anemia, severe malignant malnutrition, hemorrhagic pancreatitis, aortic valve replacement, hemorrhagic fever, severe nephrotic syndrome, erythropoiesis protoporphyrin, and liver disease (eg subacute hepatic necrosis, Cirrhosis, chronic active hepatitis, primary liver cancer, etc.). Among them, the degree of HPx reduction in liver disease is parallel to liver damage.

Normal value:
Heme binding protein (serum adult): 0.50-1.15g/L

Above normal:
Elevated serum levels HPx is a mildly reactive acute phase response protein. In acute stress response, the HPx concentration does not rise more than twice as much as normal. Certain tumors can also be elevated, especially melanoma.

negative:

Positive:

Tips: Before the examination, the diet is light and alcohol is prohibited. Check for an empty stomach in the morning. Normal value

Immunodiffusion

Serum adults ranged from 0.50 to 1.15 g/L with an average of 0.75 g/L.

Full-term fetus > adult concentration 30%, or > maternal 18%.

The mother is 1.5 times higher than the non-pregnant woman.

The mean urine value was 2.0 mg/24h.

Clinical significance

1. Increased serum levels HPx is a mildly reactive acute phase reaction protein. In acute stress response, the HPx concentration does not increase by more than 2 times that of normal rest. Certain tumors can also be elevated, especially melanoma.

2, serum levels decreased in severe hemolytic anemia, severe malignant malnutrition, hemorrhagic pancreatitis, aortic valve replacement, gray fever, severe nephrotic syndrome, erythropoiesis protoporphyrin and liver disease (such as subacute liver Necrosis, cirrhosis, chronic active hepatitis, primary liver cancer, etc.). Among them, the degree of HPx reduction in liver disease is parallel to liver damage.

Low results may be diseases: acute hemorrhagic necrotic pancreatitis, acute stress response, primary liver cancer, malignant melanoma results may be high disease: hemolytic anemia considerations

HPx can be combined with globin (Hp) to treat hemoglobin (Hb). When Hp is depleted during extensive hemolysis, free Hb can be degraded into globin and heme, while heme is insoluble in water and can form a complex with HPx. Through the blood flow to the liver decomposition, iron can be reused by the body, effectively preventing the loss of iron. The heme-degraded porphyrin ring is further metabolized to bilirubin excreted by the bile, which in turn is repeatedly utilized in the blood circulation.

Continuous dynamic observation of changes in serum HPx to understand intravascular hemolysis, its clinical value is better than Hp, because in the case of hemolysis, serum Hp levels can be reduced to zero. For example after an extracorporeal circulation surgery. The continued decline in HPx levels indicates that red blood cells continue to destroy.

Inspection process

Reagent

1, serum: should be fresh serum to avoid interference between hemolysis and lipids. When it cannot be measured in time, it is stable at 72 °C for 4 hours, -20 °C for 6 months, and -70 °C for long-term storage.

2. Urine: Take fresh urine for 24 hours. After centrifugation, take the supernatant and adjust the pH to 7.0 for analysis. It can also be stored in a low temperature refrigerator at -20 °C for 1 year.

Method of operation

The same as the immunodiffusion method.

Not suitable for the crowd

no.

Adverse reactions and risks

no.