Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate and ADP to pyruvate and ATP. It is one of the main rate-limiting enzymes in the glycolysis process. There are two isoforms, M and L. M type has M1 and M2 subtypes. M1 is distributed in the myocardium, skeletal muscle and brain tissue; M2 is distributed in tissues such as the brain and liver. L-form isoenzymes are mainly found in liver, kidney and red blood cells. After myocardial necrosis, PK is released into the blood, and PK can be used to diagnose myocardial infarction.

Basic Information

Specialist classification: cardiovascular examination classification: biochemical examination

Applicable gender: whether men and women apply fasting: fasting

Analysis results:

Below normal:
Patients with congenital non-spherical cell hemolytic anemia of hematological diseases have significantly reduced PK total activity in red blood cells. In other blood diseases such as acute leukemia, erythrolecular leukemia, aplastic anemia, ineffective anemia, paroxysmal nocturnal hemoglobinuria, congenital hypoplasia, etc., erythrocyte PK activity can be reduced to 50% of the control value.

Normal value:
Serum pyruvate kinase: 28.3-±12.8U/L

Above normal:
1. The activity of PK began to increase 2 h after the onset of acute myocardial infarction, the peak of PK activity at 22-24 h (3 times of the control value), the peak on the 2nd day (four times the control value); and gradually returned to normal after 48-72 h. 2. The total serum PK activity of patients with cervical cancer, lymphosarcoma, myeloid leukemia and Hodgkin's disease was significantly increased. 3, patients with myogenic diseases (dummy hypertrophy, Becker muscular dystrophy, limb band type and facial scapular muscular dystrophy), serum PK activity is significantly increased.

negative:

Positive:

Tips: In acute myocardial infarction, it increased significantly on the first day and returned to normal on the fifth day. Normal value

Serum (28.3 ± 12.8) U / L (pyruvate kinase method).

Clinical significance

1. The activity of PK began to increase 2 h after the onset of acute myocardial infarction, the peak of PK activity at 22-24 h (3 times of the control value), the peak on the 2nd day (four times the control value); and gradually returned to normal after 48-72 h. Increased serum PK activity is more specific for acute myocardial infarction. In addition, since PK activity begins to increase and returns to normal after acute myocardial infarction, it can be used for the diagnosis of acute myocardial infarction extension or reinfarction. However, some people think that PK is not as sensitive as CK, but it is more specific than CK. Because of the short-term increase, it is best to supplement it with other monitoring indicators. Some people think that PK is a simple, rapid and reliable indicator for the diagnosis of acute myocardial infarction.

2. The total serum PK activity of patients with cervical cancer, lymphosarcoma, myeloid leukemia and Hodgkin's disease was significantly increased.

3, patients with myogenic diseases (dummy hypertrophy, Becker muscular dystrophy, limb band type and facial scapular muscular dystrophy), serum PK activity is significantly increased, up to the control value of 1 ~ 25 Times, the positive rate is >90%. In contrast, in patients with neurogenic diseases (spinal muscular atrophy, amyotrophic lateral dysplasia, etc.), most people have normal serum PK activity. Therefore, determination of serum PK activity has certain value for distinguishing between myogenic or neurogenic myopathy.

4, blood system diseases congenital non-spherical cell hemolytic anemia patients significantly reduced PK total activity in red blood cells. In other blood diseases such as acute leukemia, erythrolecular leukemia, aplastic anemia, ineffective anemia, paroxysmal nocturnal hemoglobinuria, congenital hypoplasia, etc., erythrocyte PK activity can be reduced to 50% of the control value.

Low results may be diseases: leukemia, aplastic anemia high results may be diseases: myocardial infarction precautions

In acute myocardial infarction, it was significantly elevated on the first day and returned to normal on the fifth day.

Inspection process

Immediately after blood collection, the test method was determined by the pyruvate kinase method.

Not suitable for the crowd

no.

Adverse reactions and risks

no.