Inactivated serum responsiveness test
When Treponema pallidum infects the body, the damaged cells can release an antigenic lipid that stimulates the body to produce anti-lipid-like antibodies, which are non-specific anticardiolipin antibodies, ie, reactive hormones. Clinically, it is mostly used to detect non-immune antibodies for syphilis serology. This non-specific antibody titer is closely related to the clinical symptoms of syphilis. Quantitative determination can be used to observe the efficacy and prognosis.Basic Information
Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection
Applicable gender: whether men and women apply fasting: fastingAnalysis results:
Normal, not infected with syphilis.
Can be used as a reference for the diagnosis and treatment of syphilis. The initial check titer is 1:4 or more. After 2 to 4 weeks interval, the titer is increased by 2 titers or 2 times. The high titer can be used as the diagnostic basis for syphilis.
Positive can be used as a reference for the diagnosis and treatment of syphilis. The initial check titer is 1:4 or more. After 2 to 4 weeks interval, the titer is increased by 2 titers or 2 times. The high titer can be used as the diagnostic basis for syphilis.Positive results may be diseases: syphilis, third-stage syphilis, leprosy considerations
Requirements for inspection:
This test can lead to biological false positives, such as leprosy, acute infection, hepatitis, malaria, cervical cancer, rheumatoid arthritis, systemic lupus erythematosus, autoimmune hemolytic anemia. In addition, ether anesthesia can also cause false positives.
Preparation before inspection:
Inform the doctor about medication history and family history.
Need to check the crowd:
People who have had a history of unclean sexual contact.Inspection process
1. Laboratory materials: blood.
2, the principle of inactivated serum responsiveness test:
The VDRL antigen pellet was reconstituted in USR buffer. Among them, choline chloride has chemical inactivation, and ethylenediaminetetraacetic acid EDTA can protect antigen and delay its denaturation. Therefore, the serum to be tested can be used without being inactivated.
This test has a commercial kit. The USR antigen suspension in ampoules has a uniform turbidity and no impurities. 6 to 10% of the dark area can be stored for half a year.
4. Operation method:
(1) Qualitative test of slide: 0.05ml of the test serum was added to the reaction circle of the special slide and spread evenly. The calibrated flat-mouth needle (45±1 drops/ml) was used to vertically add 1 drop of USR antigen suspension to the serum in the reaction circle, and manually rotated by 180r/min for 4 minutes (if the serum is 0.03ml, the reagent is used instead) 0.013ml). The results were observed with a 10 x 10x microscope within 3 min. The results are reported in the same way as the VDRL method.
(2) Semi-quantitative test of slides: Take a row of test tubes and dilute the test serum with physiological saline. The rest of the operation is the same as the qualitative test. The highest dilution at which an agglutination reaction occurs is the serum titer of the serum.Not suitable for the crowd
Nothing is suitable for the crowd.Adverse reactions and risks
Generally no complications and harm.