Serum phospholipids mainly include four parts: lecithin, lysolecithin, neurophospholipid and cephalin. In clinical work, it is generally suspected to determine serum total phospholipids. There are two methods for chemical and enzymatic determination. For further examination of the composition of serum phospholipids, thin layer chromatography, gas chromatography or high performance liquid chromatography is required.Basic Information
Specialist classification: growth and development check classification: biochemical examination
Applicable gender: whether men and women apply fasting: fastingAnalysis results:
Reduced in severe hepatitis, decompensated cirrhosis, Tangier disease, hyperthyroidism, malabsorption syndrome, myeloproliferative disease, multiple myeloma, Wolman's disease, Leye syndrome, multiple sclerosis, etc. .
Serum phospholipid: 1.7-3.2mmol/L
Increased in obstructive jaundice, primary biliary cirrhosis, primary sclerosing cholangitis, Ziere syndrome, glycogen accumulation disease, obesity, diabetes, acute and chronic pancreatitis, nephrotic syndrome, hypothyroidism , fatty liver, fatty malnutrition, pregnancy, oral contraceptives, etc.
Enzyme rate method (37 ° C)
1.7 ~ 3.2mmol / L (130 ~ 250mg / dl) in P × 25;
1.7 to 3.2 mmol/L (5.2 to 9.9 mg/dl) in terms of P.Clinical significance
1, elevated in obstructive jaundice, primary biliary cirrhosis, primary sclerosing cholangitis, Ziere syndrome, glycogen accumulation disease, obesity, diabetes, acute and chronic pancreatitis, nephrotic syndrome, thyroid function Reduced symptoms, fatty liver, fatty malnutrition, pregnancy, oral contraceptives, etc.
2, reduced in severe hepatitis, decompensated cirrhosis, Tangier disease, hyperthyroidism, malabsorption syndrome, myeloproliferative diseases, multiple myeloma, Wolman disease, Leye syndrome, multiple sclerosis Symptoms, etc.Low results may be diseases: liver, pancreas trauma, multiple myeloma results may be high disease: obesity, lipodystrophy, malabsorption syndrome, thyroid hyperthyroidism in children
1, phospholipase D hydrolysis specificity is not high, but serum lecithin, lysolecithin and neurophospholipids account for about 95% of total phospholipids, so the measured value can represent serum total phospholipids.
2, the above enzyme formula is for reference only, stabilizers and additives need to be studied.Inspection process
Immediately after blood collection, the test was carried out. In the 3.0 ml enzyme application solution, 20 μl of serum, 20 μl of standard solution, 20 μl of water and 20 μl of water were added, mixed, and kept at 37 ° C for 10 min, and the color was measured at a wavelength of 500 nm.
The method uses phospholipase D to hydrolyze lecithin, lysolecithin and neurophospholipid, and the choline produced is oxidized by choline oxidase to form hydrogen peroxide, which can be color-developed by Trinder reaction.Not suitable for the crowd
no.Adverse reactions and risks