Aldolase (ALD or ALS) is a fructose-1,6-diphosphate D-glyceraldehyde-3-phosphate lyase. ALD is widely distributed in various animal tissues, among which skeletal muscle has the highest vitality, followed by brain, heart and liver, and serum is the lowest. The enzyme activity in red blood cells is 15 times higher than serum. The ALD assay mainly has a colorimetric method and an enzyme coupling method.Basic Information
Specialist classification: Digestive examination classification: biochemical examination
Applicable gender: whether men and women apply fasting: fastingAnalysis results:
Found in fructose intolerance, Tay-Sachs disease (cerebral macular degeneration syndrome).
1 significantly increased in polymyositis, progressive muscular dystrophy, congenital muscular dystrophy, extensive skeletal muscle injury, occlusive motility, phlebitis, acute hemorrhagic pancreatitis, pulmonary infarction, fever, severe burns, carbon monoxide poisoning, Myocardial infarction with heart failure, prostate cancer, etc. 2, mild rise in the limbs of muscular dystrophy, facial scapula, wrist, muscle tension, hyperthyroidism, and reduction, primary or secondary liver cancer, leukemia (FDP/FIP in leukemia) The ratio increased, the muscle atrophy syndrome, chronic hepatitis, cerebrovascular disease and the like increased slightly.
1. The colorimetric method is 5 to 27 U/L for adults, 4 times for newborns, and 2 times for children.
2, enzyme coupling method male 2.61 ~ 5.71U / L. Female 1.98 ~ 5.54U / L.Clinical significance
Serum ALD assays are primarily used to diagnose muscle and liver disease.
1, muscle disease, muscular dystrophy, polymyositis and other patients with elevated serum ALD activity. In muscular dystrophy, the enzyme activity of the large scale, limb band and distal type is the highest, and the scapular type and the eye muscle type are only slightly elevated or normal. In general, ALD activity decreases with age, and enzyme activity increases most significantly during active phase and muscle atrophy, so its determination contributes to the diagnosis of this disease. The serum ALD activity of patients with myocardial infarction is elevated, generally reaching a peak at 24 to 48 hours after the onset of chest pain. The change rule is the same as AST, but it is earlier than AST. It is normal when angina is present.
2, liver disease acute viral hepatitis ALD activity rise and fall parallel with ALT. Chronic hepatitis, cirrhosis, and obstructive jaundice are only slightly elevated.High results may be diseases: myocardial infarction, polymyositis, pediatric pseudo-hypertrophic muscular dystrophy, muscular dystrophy, polymyositis, no myopathy dermatomyositis considerations
1. Avoid strenuous exercise before taking blood.
2, the use of cortisone, adrenocorticotropic hormone treatment, can promote the serum aldolase activity is significantly increased.Inspection process
1, colorimetric method:
10.1 mol/L Trimethylpyridine buffer (pH 7.4): Weigh 1.21 g of 2,4,6-trimethylpyridine or 1.32 ml, dilute in 50 ml of distilled water, and adjust the pH to 7.4 with 1 mol/L HCl ( About 3 to 4 ml) and diluted to 100 ml. Since trimethylpyridine is volatile, the pH should be checked frequently.
20.56 mol/L barium sulfate solution (pH 7.4): 7.29 g of barium sulfate (NH2-NH2·H2SO4) was dissolved in 60 ml of 1 mol/L NaOH, adjusted to pH 7.4, and diluted to 100 ml with water.
32 mmol/L iodine acetic acid solution (pH 7.4): 37 mg of iodoacetic acid, dissolved in 2 ml of 0.1 mol/L NaOH, and diluted to 100 ml with water.
450mmol / L 1,6-diphosphate fructose substrate solution (pH 7.4): Weigh 384mg of fructose 1,6-diphosphate disodium salt, dissolved in 10ml of distilled water, adjusted to pH 7.4 with 1mol / L NaOH, diluted with distilled water To 20ml. The refrigerator is kept for 2 weeks.
5100 g / L trichloroacetic acid.
60.75 mol / L NaOH solution.
71 g/LDNPH solution: 100 mg of DNPH was weighed, dissolved with 2 mol/L HCl and diluted to 100 mL.
82g / L dihydroxyacetone storage standard solution: accurately weigh 200mg of dihydroxyacetone, dissolved in distilled water and diluted to 100ml, placed in the refrigerator for 48 ~ 72h fully depolymerized and used.
90.2 g/L dihydroxyacetone application standard solution: The storage standard solution was diluted 10 times with distilled water.
2. Enzyme coupling method:
1 substrate buffer: weigh 220mgNa2FDP·8H2O (or 370mg tricyclic ammonia FDP·H2O), 6.2mg iodoacetic acid, dissolved in 90ml distilled water, add 0.75ml 2,3,6-trimethylpyridine, mix, use 5mol /LHCl was adjusted to pH 7.4 (about 0.6 ml) and diluted to 100 ml with distilled water. 2 to 8 ° C can be stable for 4 weeks. This solution contained 35 mmol/L of trimethylpyridine, 0.3 mmol/L of iodoacetic acid, and 4 mmol/L of FDP.
215 mmol/L NADH solution: 25 mg of Na2NADH and 20 mg of NaHCO3 were dissolved in 2 ml of distilled water and stabilized at 2-8 ° C for 4 weeks.
3GDH/TPI/LDH suspension (product of BoehfingerMannheim): diluted with 3.2 mol/L ammonium sulfate solution into a mixed enzyme solution, so that each enzyme concentration is glycerol-1-phosphate dehydrogenase (GDH)>75 KU/L, phosphoric acid Sugar isomerase (TPI) > 500 KU / L, lactate dehydrogenase (LDH) > 233 KU / L (25 ° C), 2 ~ 8 ° C can be stable for 1 year, fully mixed before use.Not suitable for the crowd
Inappropriate people: Generally there are no people who are not suitable.Adverse reactions and risks
Risk of infection: If you use an unclean needle, you may be at risk of infection.