Serum aldolase (ALD)
A type of lytic enzyme, narrowly defined as an enzyme that catalyzes the cleavage of 1.6-diphosphate-D-fructose to produce 3-phospho-D-glyceraldehyde and α-dihydroxyacetone phosphate, which means 1.6-diphosphate-D-fructose. Reduced enzyme (Ec188.8.131.52). (In a broad sense, an enzyme which catalyzes the same form reaction, for example, a rhamnose phosphate aldolase or the like is also collectively referred to as an aldolase). Clinically used mainly for the diagnosis of liver and muscle diseases.Basic Information
Specialist classification: Digestive examination classification: biochemical examination
Applicable gender: whether men and women apply fasting: fastingAnalysis results:
Found in fructose intolerance, Tay-Sachs disease (cerebral macular degeneration syndrome).
1 significantly increased in polymyositis, progressive muscular dystrophy, congenital muscular dystrophy, extensive skeletal muscle injury, occlusive motility, phlebitis, acute hemorrhagic pancreatitis, pulmonary infarction, fever, severe burns, carbon monoxide poisoning, Myocardial infarction with heart failure, prostate cancer, etc. 2, mild rise in the limbs of muscular dystrophy, facial scapula, wrist, muscle tension, hyperthyroidism, and reduction, primary or secondary liver cancer, leukemia (FDP/FIP in leukemia) The ratio increased, the muscle atrophy syndrome, chronic hepatitis, cerebrovascular disease and the like increased slightly.
Adult 5 ~ 27U.
Newborns are about four times as many as adults.
Children are about twice as many as adults.
2, enzyme rate method (37 ° C)
Male 2.61 ~ 5.71U / L, female 1.98 ~ 5.54U / L.Clinical significance
Serum ALD assays are primarily used to diagnose muscle and liver disease.
1, muscle disease, muscular dystrophy, polymyositis and other patients with elevated serum ALD activity. In muscular dystrophy, the enzyme activity of the large scale, limb band and distal type is the highest, and the scapular type and the eye muscle type are only slightly elevated or normal. In general, ALD activity decreases with age, and enzyme activity increases most significantly during active phase and muscle atrophy, so its determination contributes to the diagnosis of this disease. The serum ALD activity of patients with myocardial infarction is elevated, generally reaching a peak at 24 to 48 hours after the onset of chest pain. The change rule is the same as AST, but it is earlier than AST. It is normal when angina is present.
2, liver disease acute viral hepatitis ALD activity rise and fall parallel with ALT. Chronic hepatitis, cirrhosis, and obstructive jaundice are only slightly elevated.High results may be diseases: polymyositis, myocardial infarction, pediatric pseudo-hypertrophic muscular dystrophy, muscular dystrophy, polymyositis, no myopathy dermatomyositis
1. Avoid strenuous exercise before taking blood.
2, the use of cortisone, adrenocorticotropic hormone treatment, can promote the serum aldolase activity is significantly increased.Inspection process
Enzyme coupling method:
1 Take 2.5 ml of substrate buffer, 0.05 ml of NADH solution, 0.01 ml of GDH/TPL/LDH mixed enzyme solution, 0.2 ml of serum or plasma, and place in a cuvette.
2 mixed, incubated at 37 ° C for 5 min.
3 Read the absorbance A1 at a wavelength of 340 nm.
After an accurate incubation at 437 ° C for 20 min (not counting 5 min in the second step), the absorbance A2 was read at a wavelength of 340 nm.
5 If △A(A1-A2)>0.50, the specimen was diluted 5 to 10 times with isotonic saline and retested.
1 LDH was added to the reaction system to remove the interference of endogenous pyruvic acid. The linear range of this method is at least 180 U/L.
2 specimens should not be hemolyzed, the serum can be stored at room temperature for 48 hours, and stored at 4 °C for 3 to 4 weeks, the enzyme activity has no significant change.
The 3DDP measured the highest ALD activity between 4 and 5 mmol/L, and the higher substrate concentration could be inhibited.Not suitable for the crowd
Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the test, and patients with a history of drug administration in the near future are prohibited.Adverse reactions and risks
Discomfort: There may be pain, swelling, tenderness, and visible subcutaneous ecchymosis at the puncture site.