Serum sorbitol dehydrogenase

Sorbitol dehydrogenase (SDH), also known as Iditol dehydrogenase (ID), reversibly catalyzes the oxidation of D-sorbitol to D-fructose. The enzyme in the human body is mainly distributed in the liver, and the content in the kidney, brain, heart, spleen and other tissues is extremely small, and the activity of the enzyme in the serum is very low, such as an increase in enzyme activity, which strongly suggests liver damage. Detection of serum SDH may be more specific than ALT in reflecting liver damage.

Basic Information

Specialist classification: Digestive examination classification: biochemical examination

Applicable gender: whether men and women apply fasting: fasting

Tips: Specimens avoid hemolysis, and check immediately after blood collection. Normal value

Healthy adults (n=60) serum 0-3.1 U/L.

Clinical significance

1. Most of the SDH in normal human serum cannot be detected.

2. Serum SDH activity was significantly increased in acute hepatitis. It is generally believed that SDH is often elevated in the early stage of jaundice or early hepatitis in acute hepatitis, peaking after 1 week of onset, and the increase is roughly balanced with transaminase. The time to return to the normal range is earlier than that of transaminase (ALT, AST). Therefore, it is of great value for the early diagnosis of hepatitis.

3, in obstructive jaundice and other diseases, generally do not rise, even if occasionally elevated, it is also due to liver damage. Therefore, it can reflect liver damage earlier, sensitively and specifically, especially for hepatocyte jaundice and obstructive jaundice.

4. The activity of this enzyme in the chronic phase of chronic hepatitis and cirrhosis is in the normal range, and the activity period is slightly increased. This enzyme activity can be increased when the cancer is metastasized.

5. When endotoxin shock causes hypoxemia and hepatic ischemia, the central lobular cells of the liver are severely damaged, and the activity of the enzyme is significantly increased.

High results may be diseases: chronic hepatitis, cirrhosis, liver damage precautions

1. The linear range of this method is 0-180U/L. Intra- and inter-batch CV were 3.9% and 5.2%, respectively.

2. Adding 2 mmol/L zinc ion to the reaction reagent can increase the enzyme activity by 10%.

3. The concentration of glycine buffer was 0.1mol/L, and the activity of SDH was the highest when the pH was 9.5~10.0.

Inspection process

Immediately after venous blood collection, the test method:

Reagent A5μl was mixed with serum 50μl, placed in a 37°C water bath for 5min, and then added 1ml of reagent B pre-warmed to 37°C, quickly mixed and transferred to a 37°C thermostat cuvette (10mm optical path) and monitored at 340nm. After the delay time of 60 s, the absorbance was read once every 30 s, and the reading was continued for 3 min, and the average change in absorbance per minute (ΔA/min) was obtained. Under the above conditions, the amount of enzyme which catalyzes the production of 1 μmol of NADH per minute is defined as one unit of enzyme activity.

Not suitable for the crowd

Inappropriate people: Generally there are no people who are not suitable.

Adverse reactions and risks

Discomfort: There may be pain, swelling, tenderness, and visible subcutaneous ecchymosis at the puncture site.