Serum acid phosphatase
Acid phosphatase (ACP) is a group of phosphatases with an optimum pH below 7. It is the most active at pH 5 and can hydrolyze various orthophosphoric acid monoesters. ACP is present in various tissues and body fluids, such as prostate, liver, spleen, kidney, red blood cells, plasma, milk, saliva, etc. About 1/3 to 1/2 of ACP in healthy male serum is derived from the prostate. The ACP assay is primarily a colorimetric method.Basic Information
Specialist classification: cardiovascular examination classification: biochemical examination
Applicable gender: whether men and women apply fasting: fastingTips: The serum should be separated in time, the specimen can not be hemolyzed, otherwise the measurement result is high. Normal value
Male total ACP 2.4 ~ 5.0U / L, PAP <1.2U / L.
Female total ACP is roughly the same as male.Clinical significance
ACP assay is mainly used for the auxiliary diagnosis of prostate cancer.
1, prostate cancer, especially metastatic cancer ACP is significantly elevated. PAP is more sensitive to ACP than ACP. Both of them are more valuable for the diagnosis, treatment and prognosis of advanced prostate.
2, blood diseases such as granulocyte leukemia, high snow disease, Niemann's disease, primary thrombocytopenic purpura, hemolytic anemia and other ACP activities are also increased.
3, non-malignant prostate disease prostatitis, prostatic hypertrophy, prostate infarction and other ACP activities are also increased.
4, bone disease, osteoarthritis, osteogenesis imperfecta, rickets, osteosarcoma, multiple myeloma and some non-prostate malignant tumor bone metastasis, ACP activity can also be elevated.
5, other hyperthyroidism, acute and chronic nephritis, urinary retention and other ACP activity can be increased.High results may be diseases: elderly patients with prostate cancer, prostate cancer, prostatitis, benign prostatic hyperplasia, secondary thrombocytosis
1. Serum and heparin anticoagulated plasma results were similar, and oxalate anticoagulated plasma results were low.
2, the serum should be separated in time, the specimen can not be hemolyzed, otherwise the measurement results are high.
3. The specimen is extremely unstable in the higher temperature and alkaline environment. If it cannot be measured in time, the serum should be frozen or acidified and stored in the refrigerator for one week. Serum acidification method: 1 ml serum plus 50 μl of 5 mol/L acetate buffer can reduce the pH to 5.4.Inspection process
Operate according to Table 1.
Mix, 37 ° C for 5 min, 405 nm with distilled water to zero, 1 cm optical path, read absorbance of A1, then set 37 ° C after 5 min, the colorimetric is A2. A2-A1 is the absorbance change value of the enzymatic reaction for 5 min.
If measured by automatic analyzer, it can be programmed according to the main parameters: wavelength 405nm, 37 °C, sample to reagent volume ratio is 1:15, delay time 5min, linear time 10min, kinetic method.Not suitable for the crowd
Inappropriate people: Generally there are no people who are not suitable.Adverse reactions and risks
1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection.
2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.