Serum acid phosphatase isoenzyme

Acid phosphatase (ACP) has 20 isoenzymes. The clinically determined isozymes are roughly two types. One is prostatic acid phosphatase (PAP), which is the most active acid phosphatase isoenzyme in the body. One class is non-prostatic acid phosphatase. There are many methods for measuring prostatic acid phosphatase, such as chemical method, radioimmunoassay, immunoprecipitation, convection immunoelectrophoresis, and electrophoresis. Non-prostatic acid phosphatase is generally still chemically used.

Basic Information

Specialist classification: cardiovascular examination classification: biochemical examination

Applicable gender: whether men and women apply fasting: fasting

Tips: Before the examination, the diet is light and alcohol is prohibited. Check for an empty stomach in the morning. Normal value

Electrophoretic measurement: ACP1 (-), ACP2 (-), ACP3 (+), ACP3b (-), ACP4 (-), ACP5 (+), ACP3 > ACP5, RBC-ACP (-).

Clinical significance

1. ACP2 elevation is seen in chronic myelogenous leukemia and prostate disease.

2, ACP3 elevated in lymphocytic leukemia, thrombocytopenia, thrombocytopenia (caused by increased platelet destruction).

3. ACP3b elevation is seen in acute and chronic granulocyte leukemia.

4. ACP4 elevation is seen in monocytic leukemia.

5, ACP5 elevated in giant cell leukemia, hemolytic anemia, giant cell anemia, sickle cell anemia, hyperthyroidism.

High results may be diseases: prostatic disease, acute lymphocytic leukemia, chronic myeloid leukemia, essential thrombocytopenia precautions

1, colorimetric method:

1 The matrix solution should not contain free phenol. If the blank tube is red, it indicates that the phenyl phosphate disodium has been decomposed and should be reconstituted.

2 Potassium ferricyanide should be stored in the dark, and blue will be ineffective. After the addition, it should be mixed quickly to fully develop color.

3 Hemolysis and jaundice serum should be used as a self-control tube to clear the error.

2. Continuous monitoring method:

1 should be taken on an empty stomach. A high-fat diet can increase the synthesis of ALP in the small intestine, a high-sugar diet can also increase the enzyme activity, and a high-protein diet can reduce the enzyme activity.

2 serum and heparin anticoagulated plasma results were consistent. The activity of the hemolytic specimen enzyme is decreased, and the anticoagulant of the complex enzyme can inhibit the enzyme activity.

3 temperature can affect the measurement results. The enzyme activity can be increased at room temperature and in the refrigerator, and the enzyme activity is increased by 3% to 6% for 1 to 4 days. The enzyme activity of the frozen serum is reduced, and the recovery is resumed after rewarming, so the specimen is preferably fresh and timely measured.

4 The type of buffer and the purity of the reagent can affect the measurement results. Different types of buffers have different reference intervals. It is used as a standard for the correction of the molar absorptivity - p-nitrophenol and phosphoric acid p-nitrophenol used as a substrate must meet certain specifications.

Inspection process

Immediately after venous blood collection, the test method was determined by electrophoresis.

Not suitable for the crowd

Inappropriate people: Generally there are no people who are not suitable.

Adverse reactions and risks

Discomfort: There may be pain, swelling, tenderness, and visible subcutaneous ecchymosis at the puncture site.