Serum free glycerol

45% of the fatty acids in the blood are formed by esterification with glycerol. If the serum free fatty acid concentration is greater than 2 mmol/L, it can be appropriately diluted and then measured.

Basic Information

Specialist classification: Digestive examination classification: biochemical examination

Applicable gender: whether men and women apply fasting: fasting

Tips: Before the examination, the diet is light and alcohol is prohibited. Check for an empty stomach in the morning. Normal value

3 to 10 years old, 65 to 228 μmol/L (0.6 to 2.1 mg/dl).

>11 years old 33 ~ 185μmol / L (0.3 ~ 1.7mg / dl).

Clinical significance

Elevation can be seen in hyperthyroidism, chronic renal failure, diabetes, cirrhosis, hyperlipidemia, and obesity.

High results may be diseases: obesity, hyperlipidemia, chronic renal failure, cirrhosis

If the serum free fatty acid concentration is greater than 2mmol / L, it can be diluted after appropriate dilution.

Inspection process

Immediately after venous blood collection, the test operation:

1. Mixing reagents:

1 Reagent I: buffer solution I4.0ml, ATP2.0ml, MgCl22.0ml, coenzyme A2.0ml, TritonX-1004.0ml, acyl-CoA synthetase 2.0ml, and double distilled water 4.5ml (20.5ml total).

2 reagent II: containing MES buffer 10.0ml, 4-aminoantipyrine 6.0ml, TBHB8.0ml, NaN3 solution 2.0ml, TritonX-1004.0ml, peroxidase solution 0.4ml, double distilled water 10.0ml (total 40.4ml).

3 Reagent III: 2.1 ml of NEM buffer, 2.0 ml of acyl-CoA oxidase (4.1 ml in total).

The above three mixed reagents can be stable for 3 days at 4 ° C in the dark.

2. Add 1 ml of reagent and 2 ml of reagent II to each tube and mix at 37 ° C for 10 min.

3. Add 50 μl of blank and serum samples, mix at 37 ° C for 5 min, and measure the absorbance at 546 nm to A1.

4. Add 0.20 ml of reagent III and mix. After the reaction is stopped for 20-25 minutes, the absorbance is measured at 546 nm to be A2.

Not suitable for the crowd

Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the test, and patients with a history of drug administration in the near future are prohibited.

Adverse reactions and risks

1, local subcutaneous hemorrhage: after the blood collection should be pressed for a sufficient time, especially those with bleeding tendency, so as not to cause subcutaneous oozing and bruising due to no blood coagulation.

2, infection: attention to aseptic operation during venous blood collection, so as not to cause local infection.