Serum lipid egg X
Lipoprotein-X is a special lipoprotein that occurs in obstructive jaundice and is present in low-density lipoproteins, which may be involved in phospholipid reflux in bile. Determination of serum lipoprotein-X is helpful in identifying jaundice and can be used to understand the severity of cholestasis.Basic Information
Specialist classification: Digestive examination classification: biochemical examination
Applicable gender: whether men and women apply fasting: fastingTips: Serum specimens should not be stored and should be measured on the same day. Normal value
Serum LP-X was significantly elevated in extrahepatic obstructive jaundice (LP-X>1g/L), and mildly elevated (<150mg/L) in obstructive jaundice in the liver, and normal in hepatic jaundice. Therefore, the determination of serum LP-X is beneficial to the identification of jaundice.High results may be diseases: cholestatic jaundice, jaundice hepatitis precautions
1. Serum specimens should not be stored and should be measured on the same day.
2. If LP-X exceeds 10g/L, increase the amount of ether or digest 0.1ml of ether extract.
3. The appropriate concentration of cetyltrimethylammonium bromide is beneficial to the extraction of ether from LP-X. If the amount of serum is changed, the amount can be changed proportionally.Inspection process
1. Take 0.25ml of serum to be tested in a centrifuge tube, add 0.05ml of 3.9mmol/L hexadecane trimethylammonium bromide solution and 0.5ml of ether, shake for 10min (about 200 times / min amplitude 4cm) After centrifugation at 2000 r/min for 5 min, the upper ether extract was used.
2. The following is the operation according to Table 1.
Mix well, set the water bath at 37 ° C for 10 min, 630 nm, and adjust the color of the blank tube.Not suitable for the crowd
Inappropriate people: Generally there are no people who are not suitable.Adverse reactions and risks
1, local subcutaneous hemorrhage: after the blood collection should be pressed for a sufficient time, especially those with bleeding tendency, so as not to cause subcutaneous oozing and bruising due to no blood coagulation.
2, infection: attention to aseptic operation during venous blood collection, so as not to cause local infection.