Human cytomegalovirus antibody
Cytomegalovirus (CMV) can cause congenital infections. There are primary or recurrent infections during pregnancy, and CMV can infect the fetus through the placenta. The clinical manifestations are giant cell inclusion disease, sick children with hepatosplenomegaly, jaundice, thrombocytopenic purpura and hemolytic anemia, a few congenital malformations, mental retardation, neuromuscular dyskinesia, deafness, choroidal retinitis, etc. Abortion or stillbirth. Symptoms of perinatal infection are mild. Child or adult infections are transmitted through close contact and sexual contact. Transfusion infection can cause heterophilic and EBV antibody-negative infectious mononucleosis and hepatitis. People with low immunity, such as tumors, organ transplants, AIDS, etc., can cause latent CMV activation, leading to severe CMV infection, manifested as interstitial pneumonia, hepatitis, and can lead to death. In addition, CMV may also have a carcinogenic effect. Its laboratory tests include virus isolation, PCR and serological tests.Basic Information
Specialist classification: Infectious disease examination and classification: immunological examination
Applicable gender: whether men and women apply fasting: fastingAnalysis results:
Positive positive in mononucleosis, fetal premature birth, fetal malformation, intrauterine growth retardation, neonatal jaundice, organ transplants and AIDS patients cause severe infection syndrome.
The normal value is negative.Clinical significance
Positive mononucleosis, premature fetal delivery, fetal malformation, intrauterine growth retardation, neonatal jaundice, organ transplants, and AIDS patients cause severe infection syndrome.Positive results may be diseases: congenital giant cell inclusion disease, neonatal cytomegalovirus infection precautions
Infected cytomegalovirus in the population is more common, more than 90% of young people have had infection, and CMV-IgG positive antibodies can be detected. CMV-IgM positive indicates that the patient has a recent cytomegalovirus infection, but should pay attention to the specific analysis in combination with clinical conditions.Inspection process
First, the material: blood
Second, the principle of human cytomegalovirus antibody determination:
1. HCMV-infected cells (antigen) + serum to be tested + fluorescein-labeled anti-human IgG antibody → intracellular fluorescence "+" positive "-" negative.
2. False positive: HCMV can induce IgGFc receptor in infected human fibroblast plasma. It appears 24 to 48 hours after infection, and most obvious at 72 to 96 hours. It is a dim cytoplasm and dense perinuclear fluorescence, which can form a false Positive. The diluted serum to be tested can be used at a ratio of 1:40 or more, and the Fc-free IgG antiserum can be used to reduce or eliminate it by a monkey CMV or the like.
Third, the reagent
High-dose (MOI) HCMV was inoculated into monolayer cells. After about 5-7 days, when CPE reached 90%, infected cells were collected by EDTA-pancreatin digestion; mixed with uninfected cells prepared by the same method at 3:1 to provide a control. Smear, about 2 × 104 cells per well, air dried, fixed in cold acetone for 10 min, stored at -70 °C.
Fourth, the operation method
The serum was diluted with PBS. The membrane was added to the wells of the prepared antigen, and the cells were shaken at 37 ° C for 60 min, PBS was shaken for 3 min × 3, fluorescein-anti-human IgG antibody was added, 37 ° C for 30 min, PBS was shaken for 3 min × 3, 0.2 g. /L Evans Blue contrast staining.Not suitable for the crowd
Taboo population: Not clear at the moment.Adverse reactions and risks
Generally no complications and harm.