Amniotic fluid and urine identification test

If you suspect that premature rupture of membranes can not be diagnosed, you can do the pH and smear of vaginal fluid to check whether there are amniotic fluid components (crystals and fat cells) to determine whether it is amniotic fluid. Secondly, the clinical common urine protein qualitative test method was used for identification. When the amniotic fluid breaks prematurely, many times the mother often thinks it is wet and wet underwear. I don't know if the amniotic fluid is broken. However, as soon as possible, amniocentesis and urine identification test to determine the premature rupture of amniotic fluid is very important, can prevent bacteria from vaginal up to the uterus to infect the fetus, to avoid complications such as umbilical cord prolapse.

Basic Information

Specialist classification: maternity check check classification: biochemical examination

Applicable gender: whether women are fasting: not fasting

Analysis results:

Below normal:

Normal value:

Above normal:

Negative is urine.

Positive is seen in the rupture of amniotic fluid.

Tips: Amniocentesis is usually performed in the second trimester (16-21 weeks of gestation). Normal value

Amniotic fluid: Protein and sugar are qualitatively positive.

Urine: Protein and sugar are qualitatively negative.

Clinical significance

Positive: amniotic fluid rupture.

Negative: urine.

Positive result may be disease: abortion precautions

1, the urine appears turbid after heating, such as salt turbidity disappeared after boiling in acid; if the protein turbidity does not disappear.

2. When too much acid is added to keep the pH away from the isoelectric point of the protein, the protein particles get a false increase in charge.

3. When the electrolyte content in the urine of patients with salt is too small, the protein precipitation after boiling and acid addition is not obvious. It is necessary to add 1 to 2 drops of saturated NaCl solution and then measure by heating acetic acid method.

4. There are few interference factors in this method, and the detection sensitivity is 0.15g/L.

Inspection process

Amniotic fluid specimen:

Amniocentesis is usually performed in the second trimester (16-21 weeks of gestation). The urine should be drained before surgery, with both hands on the hips and gently turn the waist and abdomen. Then supine, use B-ultrasound to detect the positioning, select the puncture point, and puncture under strict aseptic operation conditions. Generally, about 20 ml of amniotic fluid is taken and placed in a clean and sterilized centrifuge tube for immediate inspection.

Urine specimen:

When using urine, use the clean container provided by the hospital and check it within 30 minutes after the urine is kept. Therefore, the medical examiner should keep urine in the hospital to prevent the urine from being too long or the container is not clean, which may affect the urine test result. Do not drink plenty of water before leaving the urine, so as not to dilute the urine, affecting the number of cells. Adults should take the middle part of the urine for examination after taking the urine.

Detection operation:

1. Take 1 large test tube and add urine to the height of 2/3 test tube.

2. Hold the test tube on the alcohol lamp and wait until the upper urine is boiled.

3. Add 2 to 3 drops of 5% acetic acid solution, gently shake, and then heat to boiling, and observe the results immediately on a black background.

Heating can denature and solidify the protein, and dilute acetic acid to make it close to the isoelectric point of albumin to promote protein precipitation. At the same time, the addition of acid can also dissolve alkaline salts.

Not suitable for the crowd

Inappropriate people: Generally there are no people who are not suitable.

Adverse reactions and risks

1, maternal injury: puncture needle stab wound blood vessels caused by abdominal wall hematoma uterine subserosal hematoma. Occasionally, amniotic fluid enters the maternal blood circulation from the puncture hole and causes amniotic fluid embolism. The bladder was not emptied before the puncture, and the bladder was injured.

2, damage to the fetus, placenta and umbilical cord: puncture needle damage to the fetus can occur bleeding, stab wounds and umbilical cord can also occur bleeding or hematoma. Therefore, the source of bleeding should be identified when taking hemorrhagic amniotic fluid. If you suspect that you are from a fetus, you should continue to listen to the fetal heart.

3, amniotic fluid leakage: postoperative amniotic fluid leakage from the needle hole, resulting in too little amniotic fluid, affecting fetal development, and even cause miscarriage or premature birth.