Human immunodeficiency virus antibody
Infection with human immunodeficiency virus causes AIDS, the acquired immunodeficiency syndrome (AIDS). There are two types of this virus, HIV-I, which is globally prevalent; HIV-II is mainly prevalent in Africa. The conventional method is to measure HIV antibodies, and the detection method is initially screened by enzyme-linked immunosorbent assay (ELISA), gelatin particle agglutination test (PA), and Western blotting (WB) can be confirmed.Basic Information
Specialist classification: Infectious disease examination and classification: blood examination
Applicable gender: whether men and women apply fasting: fastingAnalysis results:
Serum screening test (enzyme-linked immunosorbent assay, gelatin particle agglutination test) is positive, must be confirmed (Western blot test), if the test is also positive, can be diagnosed as human immunodeficiency virus infection.
Serum screening test (enzyme-linked immunosorbent assay, gelatin particle agglutination test) is positive, must be confirmed (Western blot test), if the test is also positive, can be diagnosed as human immunodeficiency virus infection.Positive results may be diseases: AIDS precautions
HIV infection can also be detected by PCR. Positive results should be combined with other examination items and clinical conditions for comprehensive analysis. Suspicious results should be followed up for at least 6 months.Inspection process
1, according to the material: blood.
2. Principle of determination of human immunodeficiency virus antibody:
ELISA method: The reaction plate was coated with genetically engineered or synthetic polypeptide antigen (containing HIV-gp41, p24, HIV-2gp36). When an anti-HIV antibody is present in the sample to be tested, it binds to the immobilized antigen. The enzyme-labeled anti-IgG is bound to the antigen, and finally the substrate is colored.
Reagents qualified by the China National Institute for the Control of Pharmaceutical and Biological Products must be used. Including: polypeptide antigen coated reaction plate, washing solution, sample diluent, HRP-rabbit anti-human IgG (γ chain), enzyme-labeled antibody dilution and substrate solution.
4. Operation method:
Follow the kit instructions. Where the sample to be tested is positive by ELISA, it must be resampled, double-well repeated measurement, still positive, and is considered positive by HIV-1/2 antibody ELISA. When using this method for large-scale testing, there are often about 1% false positives. Specimens that are ultimately judged to be positive for the enzyme's immune response must be sent to a validation laboratory approved by the national health department for confirmation in a sensitive and more specific manner.Not suitable for the crowd
It is not clear at the moment.Adverse reactions and risks
Generally no complications and harm.