Apolipoprotein CIII

Apolipoprotein C is the major apolipoprotein of very low density lipoprotein cholesterol. It is also present in high density lipoprotein-cholesterol and low density lipoprotein-cholesterol. There are 3 different apolipoproteins C, namely ApoCI, ApoCII, ApoCIII, which is a small amount of structural protein of CM, VLDL and HDL. Immunoturbidimetric assay: serum ApoCIII is combined with a specific anti-human ApoCIII antibody in the reagent to form an insoluble immune complex, which makes the reaction solution turbid. The absorbance is measured by a luminometer at a wavelength of 340 nm, representing the degree of turbidity and the degree of turbidity. Reflects the amount of ApoCIII in the serum sample, which can be read from the calibration curve of the calibrated serum.

Basic Information

Specialist classification: cardiovascular examination classification: biochemical examination

Applicable gender: whether men and women apply fasting: fasting

Tips: Before you check, pay attention to rest and ban diet. Normal value

The one-way diffusion-free method is 90 to 164 mg/L.

Clinical significance

Elevation is seen in hyperlipidemia.

High results may be diseases: precautions for hyperlipidemia

Before inspection:

1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results.

2. Fasting for 12 hours before taking blood, taking fresh blood for inspection.

When checking:

When you draw blood, you should relax your mind, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection.

After inspection:

1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma.

2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended.

3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved.

4. If there is localized congestion, use a warm towel after 24 hours to promote absorption.

Inspection process

Rocket electrophoresis:

1 pouring plate: 10g/L agarose 10ml per plate, melt in boiling water bath, mix, cold to 50 ~ 55 ° C when adding apoAI antiserum 50μl, apoB antiserum 8μl (amount depending on antiserum titer) Mix quickly and pour on the glass plate preset on the water platform, cool and place at 4 ° C, and then punch holes after 20 min. The hole is at the cathode end of the plate, the hole diameter is 3 mm, the hole spacing is at least 5 mm, and the hole volume is 5 μl. Place the plate in the electrophoresis tank and bridge with filter paper.

2 diluted antigen: the calibration serum was diluted to 1:100, 1:150, 1:200, 1:300, and 1:400 (for calibration curve) with 0.15mol/L NaCl solution, and the serum sample was diluted 1:200. Put the refrigerator at 4 °C for no more than 3 days.

3 Loading: In the low current state (10 mA / plate), dilute the fixed serum and specimens to absorb 5 μl (accurate), and add to the agarose gel sample well. Each board has to do a series of standards.

4 power: steady flow 24mA / board, terminal voltage 6 ~ 8V / cm, cooling with running water to maintain agarose 15 ° C, electrophoresis 3 ~ 4h.

5 deproteinized protein and dry film: the agarose plate after electrophoresis was immersed in 0.15mol/L NaCl for 30min, and the film was placed on the polyester film, and the glue was absorbed by the multi-layer filter paper under gentle pressure. Moisture, then dry the filter paper, film and polyester film together, or dry it with a hot air blower. After drying, the film will naturally separate from the filter paper and polyester film.

6 staining: The agarose film was immersed in the staining solution for 20 to 30 minutes.

7 Decolorization: Soak the dyed film with a decolorizing solution until the rocket peak is clear, and the background is basically colorless. It can be clamped in two pieces of cellophane in water and can be stored for a long time after drying. It can also be soaked in running water to remove the background.

Not suitable for the crowd

Inappropriate people: Generally there are no people who are not suitable.

Adverse reactions and risks

1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection.

2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.