Aerobic bacteria culture, anaerobic culture, Mycobacterium tuberculosis culture, or fungal culture can be performed as needed for the diagnosis of respiratory infections. The theoretical basis for sputum culture is that the pathogenic bacteria should be higher than the contaminated bacteria, and accordingly, the sputum is quantitatively cultured and quantitatively cultured. Often carried out with drug sensitivity tests. This test is used for a variety of respiratory infections of unknown origin.Basic Information
Specialist classification: Respiratory examination classification: sputum examination
Applicable gender: whether fasting: fastingAnalysis results:
Normally negative, that is, no pathogenic bacteria grow.
The positive is the sputum culture of the pathogenic bacteria, after which the strain can be identified and the corresponding pathogens are obtained.
Negative, that is, no pathogenic bacteria grow.Clinical significance
This test is used for a variety of respiratory infections of unknown origin.
After the sputum cultures the pathogenic bacteria, the strain identification is carried out, and the corresponding pathogens can be obtained. Common pathogens are:
1. Gram-positive bacteria include Streptococcus pneumoniae, Staphylococcus aureus, Mycobacterium tuberculosis, Actinomycetes, Nocardia, Anaerococci, Corynebacterium diphtheriae and the like.
2, Gram-negative bacteria K. buckhamii, Neisseria meningitidis, Haemophilus influenzae, Klebsiella pneumoniae, large field bacteria, Pseudomonas, Legionella and so on.
3. Culture and isolation of some fungi to help diagnose the fungal infection.
4. Some viruses can be isolated by virus isolation. If the avian influenza virus H5N1 is isolated, it can be diagnosed as avian influenza.Positive results may be diseases: Haemophilus influenzae infection, bronchitis, other Salmonella infections in children, Yersinia enterocolitica pneumonia, pediatric fungal pneumonia, lobar pneumonia, pediatric Haemophilus influenza pneumonia, Pneumococci Disease, Pseudomonas aeruginosa pneumonia, Staphylococcus aureus pneumonia
1. Because cough specimens are often contaminated by bacteria colonized by the oropharynx, the isolated bacteria often do not truly represent the pathogens of lower respiratory tract infections. They should be closely combined with clinical analysis and cultured as many times as necessary.
2. Cytological screening of sputum: light microscopy after direct smear of sputum, less than 10 squamous epithelial cells per low power field, more than 25 white blood cells, or squamous epithelial cells: white blood cells <1:2.5, It is considered that the sputum is derived from the lower respiratory tract, which is a qualified sputum specimen.
3. Due to the heterogeneity of sputum, the specimen should be liquefied and homogenized before quantitative culture.
4. Those who have used antibacterial drugs before sampling, the detected concentration of infected bacteria is reduced, which can lead to false negative results.Inspection process
The sputum specimens were collected and sent for examination, and cultured by bacterial culture method.Not suitable for the crowd
Inappropriate people: Generally there are no people who are not suitable.Adverse reactions and risks
Generally, this examination does not have too many adverse reactions and does not have much influence on the body.