The mumps virus belongs to the family Paramyxoviridae and has a structure similar to the parainfluenza virus. Mumps virus causes mumps in humans, often with orchitis and oophoritis, but also with meningitis, myelitis and pancreatitis, myocarditis, mastitis and so on. Common methods for serological diagnosis of mumps virus are ELISA, hemagglutination inhibition, complement fixation test, etc., for antibody detection.

Basic Information

Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection

Applicable gender: whether men and women apply fasting: fasting

Analysis results:

Below normal:

Normal value:
no

Above normal:

negative:
normal.

Positive:
Positive indications may be epidemic mumps, with meningitis, orchitis, pancreatitis, ovarian inflammation and other diseases.

Reminder: cooperate with the doctor's work during the examination. Normal value

negative.

Clinical significance

Positive: mumps, complicated by meningitis, orchitis, pancreatitis, oophoritis, etc.

Positive results may be diseases: epidemic mumps, acute suppurative mumps, myocarditis considerations

If the patient's clinical symptoms are not typical, there are complications such as meningitis, encephalitis, orchitis, etc., laboratory serological examination is required.

Inspection process

(1) Hemolysin titration: Each tube was added for titration according to the composition, order, and dosage of Table 1.

Hemolysin unit: The highest dilution of hemolysin that completely dissolves red blood cells. As a result of the above table, 1:1400 dilution (0.1 ml) of hemolysin is 1 unit. For example, 2 units, that is, a 1:1200 dilution (0.1 ml) of hemolysin solution was applied.

(2) Complement titration: The complement is diluted to 1:30. According to the composition, order and dosage of Table 2, the tubes were added for titration. During the whole test, the complement should be placed in an ice water box to prevent the activity from decreasing.

Complement unit: The minimum amount of complement that can cause complete hemolysis. As a result of the above table, 1:30 complement contains 0.1 unit of 0.12 ml, and 0.24 ml contains 2 units. In order to have 2 units in 0.2 ml, the dilution can be calculated as follows:

After the complement was diluted 1:25, 2 units of complement were contained per 0.2 ml.

(3) antigen titration:

1 Place 64 tubes in a square array, ie 8 rows and 8 rows.

2 The immune serum that had been inactivated (56 ° C for 30 min) was diluted twice. Add 0.1 ml of serum 1:2 to each tube of the first row of 1 to 8, and add 1:4 diluted serum to the second row of tubes 1 to 8, so that the double-diluted diluted serum is added to the seventh row. The eighth row was serum-free and replaced with 0.1 ml saline as an antigen control.

3 Dilute the antigen in the same way as above. In the first row, 1 to 8 tubes were added with 1:2 diluted antigen 0.1 ml, and in the second row, 1 to 8 tubes were added with 1:4 diluted antigen 0.1 ml. Thus, the double diluted antigen was added to the seventh line, Eight lines were not added with antigen and replaced with 0.1 ml of saline as a serum control.

4 Add 0.1 ml of saline to each control tube.

5 Then, add 0.2u of 2u complement to each tube, place the refrigerator at 4°C for 16-18h, and place it in the 37°C water bath for 30min the next day. Add 1.25% sheep red blood cells and hemolysin (2u) 0.1ml to each tube. Place in a 37 ° C water bath for 30 min.

6 observation results: such as "++++" means that the complement is completely fixed by the test system, showing no hemolysis: "-" means that the complement is completely unfixed by the test system, showing complete hemolysis; It is represented by "+++", "++", "+", and "±".

Antigen unit: The highest dilution factor of the antigen that can react with the highest dilution factor of the serum "++++" is 1 antigen unit, as shown in the table, 1:8. Two units were used for the test, namely 1:4.

Not suitable for the crowd

It is not clear at the moment.

Adverse reactions and risks

Generally no complications and harm.