Budsheng complement fixation test
A genus of genus is a pathogenic fungus. It is distributed all over the world, mainly in North America, the eastern United States, and recent reports of infections in the African continent and Israel. Earlier reports were made in the UK, the Soviet Union, and France. There have been similar reports in China, but no final culture has been identified.Basic Information
Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection
Applicable gender: whether men and women apply fasting: fastingAnalysis results:
Negative (less than 1:4).
Positive (increased titer) suggests infection with buds, coccidioides, and histoplasma.
Negative (<1:4).Clinical significance
Positive (increased titer) buds, coccidioides, histoplasmosis infection.Positive results may be diseases: bud disease, lung bud disease considerations
The test was complicated and was replaced by other methods.Inspection process
(1) Titration of complement and hemolysin.
1 Take 1ml of complement, add 9ml of BBS, and then dilute to 1:20, 1:30, 1:40, 1:50, 1:60, 1:80, 1:100, 1:200, 1:400, and take 50 0.2 ml of hemolysin, add 9.8 ml of BBS, which is 1:100 hemolysin, and then continue to dilute to 1:800, 1:1600, 1:3200, 1:6400.
2 Take the square array of test tubes, longitudinally add 0.1ml of different concentrations of complement, and add 0.1ml of hemolysin in each row (all should be added from the highest dilution), then add BBS0.2ml, complement and The hemolysin control tubes were each added with BBS 0.3 ml, and finally 2% sheep red blood cells were added to each tube. The total amount of each tube was 0.5 ml, shaken, placed in a 37 ° C water tank for 30 min, and the results were observed.
The highest dilution of complete hemolyzed complement and hemolysin is the respective unit. Hemolysin 1:3200 is a hemolysin unit, and complement 1:80 is a complement unit. In practical applications, the hemolysin is 2u (3200÷2=1600) and the complement is 2.5u (80÷2.5=32).
(2) Formal test:
1 Operate with a V-type microplate, each specimen was made into one test well and one serum control well. BBS0.025ml was added to each well.
2 Dip serum (0.025 ml) with a dilution stick to the first well, rotate and transfer to the second well for a total of eight wells, thus obtaining a 1:2 to 1:256 dilution.
Complement control tube: 2.5u = 0, 1u = 0 ~ ±, 0.5u = 3 ~ 4. Sheep red blood cell control tube: 4. Antibody control tube: 0. Negative control tube; 1:2 to 1:256=0. Positive control tube: the same positive serum, the results should be consistent.
When the reaction tube of each measuring tube is the strongest, one tube is still ± report suspicious, and 1 to 4 reports positive. If both the measuring tube and the serum control tube are anti-complement, all the measuring tubes are 0, and the report is negative.
The method of examining the antibody is the same as the method of examining the antigen, except that four units of known antigen are used instead to determine the serum to be tested.Not suitable for the crowd
Those who do not have an indication for examination should not do this check.Adverse reactions and risks
Generally, there is no complication, and infection may occur due to unclean syringe during blood drawing.