Hepatitis B virus pre-S2 antigen
The hepatitis B virus coat indicates that the antigen consists of three parts, namely the S antigen, the pre-S1 antigen and the pre-S2 antigen. The pre-S2 antigen is also an integral part of the HBV shell. The positive rate of hepatitis B in the acute phase was high, and the titer was increased; the titer in the recovery period gradually decreased. It can occur in the serum of patients with acute hepatitis B and chronic hepatitis B.Basic Information
Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection
Applicable gender: whether men and women apply fasting: fastingAnalysis results:
Prompt acute hepatitis B early and chronic hepatitis B.
The positive rate of hepatitis B in the acute phase was high, and the titer was increased; the titer in the recovery period gradually decreased. It can occur in the serum of patients with acute hepatitis B and chronic hepatitis B.Positive results may be diseases: hepatitis B virus, hepatitis B precautions
Pre-S2 protein is similar to anti-HBc-IgM in the early stage of hepatitis B.Inspection process
1, according to the material: blood.
2, Hepatitis B virus pre-S2 antigen determination principle: double antibody sandwich method: coated with microporous reaction plate with monoclonal PreS2 antibody, PreS2 in the serum to be tested with the reaction, then add HRP labeled anti-HBs to form a sandwich, and finally add The substrate is colored.
3. Reagents: There are a complete set of kits available in China. Including: monoclonal anti-PreS2 antibody; HRP-anti-HBs; horseradish peroxidase and horse anti-HBs-IgG or monoclonal anti-HBs, a combination prepared by the modified sodium periodate method.
4. Method of operation: Follow the instructions in the kit or follow the procedure: Add appropriately diluted monoclonal anti-PreS2 to the wells of the polystyrene microplate, 100 μl per well, overnight at 4 °C. Wash 3 times with distilled water. Add 100 μl of serum to be tested and set positive and negative control wells. Wash at 37 ° C for 2 h. 100 μl of diluted HRP anti-HBs was added, washed at 37 ° C for 2 h, 5 times. Add 100 μl of o-phenylenediamine-H 2 O 2 substrate solution at 37 ° C for 20 min. The reaction was terminated by adding 2 mol/L H 2 SO 450 μl. The absorbance was measured at 492 nm using an enzyme-linked detector, and P/N ≥ 2.1 was positive.Not suitable for the crowd
Generally no taboos.Adverse reactions and risks
Generally no complications and harm.