Cryptococcal complement complementation test
Cryptococusneoformans is a fungus belonging to the genus Hymenoptera, Phytophthora, Cryptococcus, Cryptococcus, and Cryptococcus. Although there are many species in this genus, it is currently considered that the main pathogenic fungus is Cryptococcus neoformans. The bacteria are widely distributed in nature and belong to soil fungi, but they are often parasitic in the feces of birds, especially pigeons. Cryptococcus neoformans is easy to invade the central nervous system. The primary tumor is mainly lung, with little skin or mucous membranes. More than 50% of patients are associated with leukemia, Hodgkin's disease, diabetes, and AIDS, and other organ transplant recipients are also susceptible to infection. Low cellular immune function is an important cause of this disease.Basic Information
Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection
Applicable gender: whether men and women apply fasting: fastingAnalysis results:
Cryptococcus neoformans infection (central nervous system and systemic).
Positive Cryptococcus neoformans infection (central nervous system and systemic).Positive results may be diseases: cryptococcal disease considerations
Some people think that the emergence of cryptococcus has certain reference value for the diagnosis of AIDS.Inspection process
(1) Titration of complement and hemolysin.
1 Take 1ml of complement, add 9ml of BBS, and then dilute to 1:20, 1:30, 1:40, 1:50, 1:60, 1:80, 1:100, 1:200, 1:400, and take 50 0.2 ml of hemolysin, add 9.8 ml of BBS, which is 1:100 hemolysin, and then continue to dilute to 1:800, 1:1600, 1:3200, 1:6400.
2 Take the square array of test tubes, longitudinally add 0.1ml of different concentrations of complement, and add 0.1ml of hemolysin in each row (all should be added from the highest dilution), then add BBS0.2ml, complement and The hemolysin control tubes were each added with BBS 0.3 ml, and finally 2% sheep red blood cells were added to each tube. The total amount of each tube was 0.5 ml, shaken, placed in a 37 ° C water tank for 30 min, and the results were observed.
The highest dilution of complete hemolyzed complement and hemolysin is the respective unit. Hemolysin 1:3200 is a hemolysin unit, and complement 1:80 is a complement unit. In practical applications, the hemolysin is 2u (3200÷2=1600) and the complement is 2.5u (80÷2.5=32).
(2) Formal test:
1 Operate with a V-type microplate, each specimen was made into one test well and one serum control well. BBS0.025ml was added to each well.
2 Dip serum (0.025 ml) with a dilution stick to the first well, rotate and transfer to the second well for a total of eight wells, thus obtaining a 1:2 to 1:256 dilution.
(3) Judgment of results: The results of each tube of the control group should meet the following conditions, otherwise it should be redone.
Complement control tube: 2.5u = 0, 1u = 0 ~ ±, 0.5u = 3 ~ 4. Sheep red blood cell control tube: 4. Antibody control tube: 0. Negative control tube; 1:2 to 1:256=0. Positive control tube: the same positive serum, the results should be consistent.
When the reaction tube of each measuring tube is the strongest, one tube is still ± report suspicious, and 1 to 4 reports positive. If both the measuring tube and the serum control tube are anti-complement, all the measuring tubes are 0, and the report is negative.
The method of examining the antibody is the same as the method of examining the antigen, except that four units of known antigen are used instead to determine the serum to be tested.Not suitable for the crowd
Those who do not have an indication for examination should not do this check.Adverse reactions and risks
Generally no complications and harm.