Specific lipase staining
The positive reaction occurs almost exclusively in the granulocytes of each stage and is more specific than POX. AS-DCE is also called granulocyte-specific esterase. The chloroacetic acid AS-D naphthol esterase in granulocytes hydrolyzes the chloroacetic acid AS-DCE naphthol in the matrix solution to produce naphthol AS-D, which is then coupled with a stable diazonium salt to form an insoluble red precipitate. In the cytoplasm.Basic Information
Specialist classification: growth and development check classification: microscopy
Applicable gender: whether men and women apply fasting: not fastingAnalysis results:
It is negative in acute monocytic leukemia and acute lymphocytic leukemia.
In acute myeloid leukemia, both myeloblasts and immature granulocytes were positive to some extent, and the activity of mature granulocytes decreased.
The positive reaction is a ruby-colored particle located in the cytosol.Clinical significance
Specific esterases, also known as granulocyte esterases, are mainly distributed in granulocytes and mast cells. The protoblasts were negative or weakly positive, and the promyelocytes and mesangocytes were strongly positive, and the granulocyte activity of the lobular cells was weakened. Eosinophils were negative, and basophils, monocytes, and mast cells were negative or weakly positive. The megakaryocytes, platelets, plasma cells, erythroid cells and lymphocytes were negative.
This staining method is mainly used to identify acute leukemia types. In acute myeloid leukemia, both myeloblasts and immature granulocytes were positive to some extent, and the activity of mature granulocytes decreased. It is negative in acute monocytic leukemia and acute lymphocytic leukemia. In acute granulocyte-monocytic leukemia, some leukemia cells (primary granules and promyelocytes) were positive, and some leukemia cells (primary mononuclear and young monocytes) were negative.Positive results may be diseases: negative results of acute granulocyte leukemia may be diseases: acute leukemia, acute lymphocytic leukemia considerations
The dyeing temperature was 38-39 ° C. At this time, the enzyme reaction was strong without obvious diffusion, and the naphthol and the fast purple sauce GBC were easily dissolved at a lower temperature.Inspection process
(1) Fixation: freshly dry smear, fixed with formaldehyde-methanol fixing solution for 30s, or with formaldehyde vapor for 5~10min.
(2) Washed and dried.
(3) Put in the working solution, 37 ° C for 30 min.
(4) Washing, hematoxylin dyeing solution for 1 ~ 2min.
(5) Washed, dried, and examined.
The dyeing temperature was 38-39 ° C. At this time, the enzyme reaction was strong without obvious diffusion, and the naphthol and the fast purple sauce GBC were easily dissolved at a lower temperature.Not suitable for the crowd
Generally there are no people who are not suitable.Adverse reactions and risks
Generally no adverse reactions.