Fecal parasites, simple examination methods, intuitive results, generally detected in defecation treatment of bowel movements. The diagnosis of intestinal parasitic diseases relies on finding eggs, protozoa trophozoites and cysts in the feces, and finding these direct evidences can clearly diagnose the corresponding parasitic diseases and parasitic infections. Large worms such as aphids, worms, and aphids or their fragments can be distinguished by the naked eye, and the hookworm worms must be washed through the sieve to see. After taking the insect repellent, you should look for the presence or absence of insects. After driving the aphids, you should carefully look for the head section.Basic Information
Specialist classification: Digestive examination classification: feces / parasitic examination
Applicable gender: whether men and women apply fasting: not fastingAnalysis results:
No eggs, protozoa trophozoites and cysts were found.
Eggs, protozoa trophozoites and cysts are found in the feces.
Negative: no eggs, protozoa trophozoites and cysts were found in the feces. No eggs, protozoa trophozoites and cysts were found.Clinical significance
(1) Non-pathogenic parasites:
1 protozoal colon amoeba, micro amoeba, eosinophilic amoeba, dinuclear amoeba.
2 flagellate, lip whipworm, Trichomonas vaginalis, Trichomonas vaginalis.
(2) Pathogenic parasites:
1 protozoal soluble tissue amoeba (encapsulated and trophozoites), Giardia lamblia (hair follicles and trophozoites).
2 helminth aphids, mites, hookworms, Schistosoma japonicum, ginger worms, whipworms, liver flukes, etc.Positive results may be diseases: tsutsugamushi disease, intestinal parasitic disease, iron worm disease, pediatric giardiasis, pouch ciliate disease, chronic amebiasis enteritis, worm, visceral visceral migration in children, tsutsugamushi disease in children And cysticercosis, insects and precautions
Requirements for inspection:
1. Prepare several smears for further inspection. The parasite eggs should be coated with thick slabs. Covers should be covered with a cover glass to avoid contamination mirrors.
2. Microscopy The purpose of microscopy is to find pathological components such as parasites and parasite eggs. The film must be observed in full film, from top to bottom, from left to right, avoiding repetition, and observing at least 10 fields per smear during microscopy. Parasites and eggs were examined with a low power microscope.
3. Pay attention to the identification of pathological components such as parasites and bacteria in the feces.
Preparation before inspection:
1. The feces with eggs are contagious and should be stored in a sealed condition.
2. The inspection of some worms needs to be carried out at night.
3, because infants and young children are not easy to get a sufficient amount of samples at a time, if you need to collect in separate parts, please temporarily store the samples in the refrigerator to avoid bacterial growth.Inspection process
(1) worm egg inspection: drop a drop of normal saline on a clean glass slide, pick a mung bean-sized fecal block with a cotton swab or toothpick, and apply it evenly in saline; the thickness of the smear can be identifiable through the smear. The writing is appropriate. Generally, it is checked under a low power microscope. If it is observed with a high power microscope, it is necessary to cover the sheet. Attention should be paid to the identification of foreign bodies in eggs and feces. The eggs have a certain shape and size; the surface of the egg shell is smooth and tidy, with an inherent color; the egg contains egg cells or larvae.
(2) Protozoan inspection:
1) Live trophozoite examination: the smear should be thin, and the method should be the same as the worm egg. The closer the temperature is to body temperature, the more obvious the activity of the trophozoites. If necessary, the holding table can be used to maintain the temperature.
2) Encapsulation of iodine staining: The direct smear method is the same as above, and one drop of iodine is used instead of physiological saline. If there is too much iodine solution, use absorbent paper to remove excess liquid from the edge of the cover slip. If you need to check the live trophozoites, you can drop a drop of iodine solution near the dung droplets that are evenly spread with saline, apply a small amount of feces in the iodine solution, and cover with a cover slip (Figure 21-1). Half of the smear staining was checked for cysts; half of the final staining was used to check the trophozoites.
3) Cryptosporidium oocyst staining examination: The current preferred method is the modified acid-fast staining method of auramine. This method can be used for fresh feces or oocyst-containing feces that are fixed in 10% formalin (within 1 month at 4 °C). The dyeing process was first stained with auramine-phenol and counterstained with modified acid-fast staining.Not suitable for the crowd
Not suitable for people:
This test is a routine check and there are no unsuitable people.Adverse reactions and risks
This test is a non-invasive test and the test itself does not present complications and hazards.