Direct bilirubin/indirect bilirubin ratio

Calculating the ratio of direct bilirubin to indirect bilirubin contributes to the type of jaundice and the identification of the disease. Astragalus is a common symptom and sign, which is caused by an increase in serum bilirubin concentration due to bilirubin metabolic disorders. Clinically, the sclera, mucous membranes, skin and other tissues are stained yellow.

Basic Information

Specialist classification: Digestive examination classification: liver function test

Applicable gender: whether men and women apply fasting: fasting

Tips: Please cooperate with the doctor when checking. Normal value


Clinical significance

(1) Elevation is seen in obstructive jaundice and acute hepatitis.

(2) Reduced in hemolytic jaundice, neonatal jaundice, and falciparum malaria.

Low results may be diseases: bile duct tumors, yellowing, viral hepatitis, high results may be diseases: children with viral hepatitis C, Rotor syndrome, biliary motility dysfunction, neonatal jaundice, pediatric acute cholecystitis, hemolytic jaundice, Malaria considerations

(1) Blood samples can be serum or plasma, and serum is more commonly used.

(2) Blood should be taken on an empty stomach to avoid hemolysis, serum should be separated as soon as possible, and stored in the dark. If it cannot be measured in time, it should be stored in the refrigerator.

(3) Long-term exposure under sunlight can significantly reduce the measurement results.

Inspection process

2 ml of fasting venous blood was taken and placed in a dry tube for examination to avoid hemolysis of the specimen.

Detection method: After fully mixing, the wavelength is 600nm, the control tube is zeroed, and the absorbance of each tube is read; or the zero is adjusted with distilled water, the absorbance of the measuring tube and the control tube is read, and the difference between the absorbance of the measuring tube and the absorbance of the control tube is used (AU- AC), the corresponding bilirubin concentration was found on the standard curve.

Not suitable for the crowd

Generally no taboos.

Adverse reactions and risks

1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection.

2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.