Cholesterol, triglyceride and lipoprotein electrophoresis are valuable for the identification of true and pseudo-chyle effusions.Basic Information
Specialist classification: Digestive examination classification: chest and ascites examination
Applicable gender: whether men and women apply fasting: fastingTips: Tell the doctor if there is pregnancy, oral contraceptives, smoking, long-term drinking, etc. Normal value
Normal value: <0.55 mmol/L (<50 mg/dl).Clinical significance
The pleural effusion triglyceride exceeded 1.24mmol / L, suggesting a chyle effusion. For example, between 0.68 and 1.24 mmol/L, further lipoprotein electrophoresis is required, and chyle effusion can be confirmed when there is a chylomicron zone.
When the triglyceride is less than 0.56 mmol/L and the chylomicron area is not obvious or absent, it is a non-chylorrhea effusion. Cholesterol effusion cholesterol level is 1.2mmol / L, malignant effusion cholesterol is greater than 1.2mmol / L, and cirrhosis ascites effusion cholesterol less than 1.2mmol / L, the sensitivity and specificity of the identification is about 90%.High results may be diseases: cirrhosis ascites, pleural effusion, cirrhosis
Requirements for inspection:
Inform the doctor if there is pregnancy, oral contraceptives, smoking, long-term drinking, etc.
Preparation before inspection:
Need to tell the doctor about the drug history.Inspection process
1. Laboratory materials: serous cavity
2, the principle of determination of ascites triglycerides:
Efficient microbial lipoprotein lipase (LPL) is used to hydrolyze TG in effluent to glycerol and fatty acids, glycerol is phosphorylated with glycerol kinase (CK) and adenosine triphosphate (ATP), and GPO is oxidized with GPO (G-3- P), then coloring with PAP reagent to form a red imine oxime compound.
(1) Dissolve the dry powder reagent according to the kit requirements using Tris buffer (pH 7.6, 150 mmol/L).
(2) The standard solution is an aqueous glycerin solution: 2.26 mmol/L.
4. Method of operation: use the enzymatic method.Not suitable for the crowd
Unsuitable for the crowd: It is necessary to check the crowd with abnormal abdominal enlargement and press the patient who can move and suspect ascites. Those without examination indications should not be tested. Hypertensive patients need to seek medical advice.Adverse reactions and risks
Infection: Pay attention to aseptic operation during puncture, pay attention to local cleaning after puncture, prevent water pollution and avoid infection.