Endocrine function tests can reflect the functional status of the endocrine glands, sub-stimulation or stimulation tests and inhibition tests. The stimulation test was performed to see if the response of the stimulated gland was normal, and the inhibition test inhibited the glandular function of the gland. Stimulating and being suppressed indicates that there are normal positive and negative feedback functions.

Basic Information

Specialist classification: gynecological examination classification: endocrine examination

Applicable gender: whether women are fasting: not fasting

Included items: Androstenedione Tips: Do not take estrogen or foods containing similar hormones. Those who are allergic to progesterone or related drugs are not suitable for this test. Normal value

1. Progesterone and female and progesterone withdraw from the blood: no abnormal blood withdrawal.

2. GnRH stimulation test: LH value increased by 2 to 3 times, peak appeared in 15 to 30 minutes (10 peptides) or 60 to 120 minutes (9 peptides).

3. Clomiphene stimulation test and dexamethasone inhibition test: negative test.

Clinical significance

Abnormal result

First, progesterone and female, progesterone withdrawal from the blood

There is withdrawal of blood to indicate that the endometrium has been prepared for estrogen and the use of progesterone causes the endometrium to turn into a secretory period and then shedding bleeding such as menstruation.

If the progesterone test is used to withdraw blood, according to other examinations, the next treatment plan can be planned; if the blood is not withdrawn, the endometrium is not prepared by estrogen or the endometrium has a lesion and does not respond. The artificial cycle of hormones and progesterone.

The artificial cycle was E21mg×28 days daily, and after 12-14 days, the progesterone was 6mg/day. There is withdrawal of blood, indicating that the endometrial response can be stimulated by exogenous estrogen and progesterone. If there is still no withdrawal from the blood, no endometrial or endometrial lesions are not responding to exogenous estrogen and progesterone, and can be diagnosed as uterine amenorrhea.

Second, GnRH stimulation test

When LH, FSH and E2 are both low, the hypothalamic GnRH deficiency or pituitary secretion should be distinguished. Intravenous injection of GnRH (10 peptide) 10?g test, blood injection 2ml before injection and 25, 45, 90 and 180 minutes after injection. The LH and FSH levels were measured, and GnRH-A (9 peptide) 5 g was used more than 10 times stronger than the 10 peptide, and the blood withdrawal time was delayed to 240 minutes.

The high peak of active response is five times higher than the baseline value, such as polycystic ovary syndrome.

The delayed reaction peak appeared later than the normal reaction time, as follows hypothalamic amenorrhea.

No response or low-fat response LH no change or slightly increased (less than 2 times), FSH changes are less, such as Xi Han syndrome, pituitary surgery or radiation damage pituitary tissue.

True precocious puberty is due to the early activation of the hypothalamic-pituitary-ovarian axis. A single measurement of gonadotropin in the blood often overlaps the pre-puberty and puberty data and does not help to differentiate between true and false precocious puberty.

Third, clomiphene stimulating test and dexamethasone inhibition test

Patients with polycystic ovary syndrome, excessive androgen, from the ovary, also from the adrenal gland. Withdrawal of blood on the 5th day with clomiphene 100mg × 5 days if the ovulation can be stimulated, androgen comes from the ovaries. When the three cycles of clomiphene citrate were ineffective, the dexamethasone suppression test was added at 0.5 mg/day to inhibit the adrenal gland and ovulate. The androgen was derived from the adrenal gland.

People who need to be examined: middle-aged women.

Positive results may be diseases: pediatric double lip syndrome, occupational acne, habitual abortion, pediatric zinc deficiency, congenital pygmy dementia syndrome in children, short stature, hypothalamic disease, baldness, early baldness

Contraindications before the test: Do not take estrogen or foods containing similar hormones.

Requirements for inspection: Checking for relaxation, checking may cause physical and psychological burdens, should be actively faced, and actively cooperate with the inspection. If the concentration of the sample to be tested is high, dilute with 0 standard solution and redo.

Inspection process

Progesterone and estrogen and progesterone withdrawal blood tests, GnRH stimulation test, clomiphene citrate test and dexamethasone inhibition test were performed in sequence.

1) Two-hour incubation method: numbered on 75mm × 12mm plastic tubes, and then operate according to the kit instructions.

2) Overnight incubation method: The preparation work is the same as above, and the operation method is different from the two-hour incubation method. Follow the kit instructions.

Not suitable for the crowd

Inappropriate people: those who are allergic to progesterone or related drugs.

Adverse reactions and risks