Fecal phosphate examination, a project in stool routine. In normal feces, a small amount of phosphate is visible. It mainly reflects the phosphate content in people's daily diet. This assay only makes sense in a particular equilibrium study. During the inspection, the polyphosphate was extracted from the feces using trichloroacetic acid, and the extract was treated with ethanol and diethyl ether, and then separated by a microcrystalline cellulose thin layer chromatography plate, and the polyphosphate was examined by spray color development.Basic Information
Specialist classification: Digestive examination classification: feces / parasitic examination
Applicable gender: whether men and women apply fasting: not fastingAnalysis results:
Below normal, it is seen in vitamin D poisoning (ie excess phosphate absorption).
Fecal phosphate: 0.4-0.8g/24h
Above normal, it is common in vitamin D deficiency.
0.4 ~ 0.8g / 24h (including organic phosphorus and inorganic phosphate).Clinical significance
1, increase vitamin D deficiency (if the dietary phosphate is appropriate), treatment with aluminum hydride (to slow down the rate of phosphate formation in kidney stones), fat sputum caused by any cause (absorption is blocked).
2, reduced in vitamin D poisoning (ie, excessive phosphate absorption). Patients who are suspected of having vitamin D deficiency need to be diagnosed and need to be diagnosed. People with other diseases need to be treated with aluminum hydride for toxicity.Low results may be diseases: high vitamin D poisoning results may be diseases: vitamin D deficiency disease considerations
Requirements for inspection:
Fresh specimens should be taken for stool inspection. The container should be clean, no urine should be mixed, and no disinfectant or sewage should be used.
This inspection needs to be completed within 1 hour, otherwise it may cause damage without formation due to the influence of pH and digestive enzymes.
Preparation before inspection:
Do not take medications containing laxatives before testing.
When dissolving the feces, the supernatant should be taken for testing.Inspection process
The polyphosphate extracted from the feces using trichloroacetic acid and the extract were treated with ethanol and diethyl ether, separated by a microcrystalline cellulose thin layer chromatography plate, and the polyphosphate was examined by spray color development.Not suitable for the crowd
Unsuitable for people: non-invasive tests, no inappropriate people.Adverse reactions and risks
This test is a non-invasive test and the test itself does not present complications and hazards.