Epstein-Barr virus is a new human virus discovered in 1964 and is the causative agent of infectious mononucleosis. Young children and adolescents are susceptible to infection. Epstein-Barr virus (EBV) is closely related to nasopharyngeal carcinoma. Nasopharyngeal carcinoma is one of the multiple cancers in southern China. Detection of EBVCA-IgA in serum is of great value in the diagnosis of nasopharyngeal carcinoma; the heterophilic antibody agglutination test of Epstein-Barr virus also has important diagnostic significance for infectious mononucleosis.

Basic Information

Specialist classification: Oncology examination classification: immune examination

Applicable gender: whether men and women apply fasting: fasting

Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. Normal value

Heterophilic agglutination test <17 (or negative) anti-EBV. IgG negative anti-EBV. IgM is negative.

Clinical significance

(1) Increase is more common in infectious mononucleosis anti-EBV. IgM showed a positive reaction, and the heterophilic antibody appeared in the blood of the patient reached a peak 3-4 weeks, and the titer exceeded 1224 to confirm the diagnosis.

(2) Anti-EBV.IgG positive has been considered in previous infection history, but anti-EBV.IgM positive can prove recent infection.

(3) Increased is also more common in children with malignant lymphoma and some patients with nasopharyngeal cancer.

(4) Serum patients and some normal people may contain a small amount of heterophilic antibodies in their blood.

Precautions

First, the precautions before blood draw:

1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results.

2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results.

3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection.

Second, after blood draw should pay attention to:

1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma.

2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended.

3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved.

4. If there is localized congestion, use a warm towel after 24 hours to promote absorption.

3. Please inform the doctor about the recent medication and special physiological changes before the test.

Inspection process

(1) NPC patient serum with a final concentration of 1:10 and 1:10 normal human serum were added to the cell smear and placed in a wet box at 37 ° C for 1 h.

(2) Dip 3 times with BSS solution for 5 min each time.

(3) A 1:10 diluted HRP-labeled anti-C3 antibody was added and placed in a 37 ° C incubator for 30 min.

(4) Wash again with BSS for 3 times.

(5) Immersion enzyme substrate (50 mg of 3,3'-diaminobenzidine dissolved in 100 ml of 0.05 mol/L pH 7.6 Tris-HCl buffer plus 30% H 2 O 2 0.1 ml) was stained.

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Adverse reactions and risks

There are no related complications and hazards.