The p53 gene is a tumor suppressor gene, which is located on the human chromosome 17p13.1 and encodes a 53kD nuclear phosphorylation protein consisting of 393 amino acids, which is called p53 protein. The p53 gene is a negative regulator of cell growth cycle and is involved in important biological functions such as cell cycle regulation, DNA repair, cell differentiation, and apoptosis. The p53 gene is divided into two types, wild type and mutant type, and its products also have wild type and mutant type. The wild-type p53 protein is extremely unstable, has a half-life of only a few minutes, and has a transactivation function and a broad-spectrum tumor suppressive effect. Mutant p53 protein has increased stability and extended half-life and can be detected by immunohistochemistry. Mutation (deletion) of p53 gene is a common event in human tumors and is related to the occurrence and development of tumors. It is generally believed that p53 overexpression is associated with tumor metastasis, recurrence, and poor prognosis.

Basic Information

Specialist classification: Oncology examination classification: immune examination

Applicable gender: whether men and women apply fasting: not fasting

Analysis results:

Below normal:

Normal value:
no

Above normal:

negative:
normal.

Positive:
P53 mutations can also be found in gastric cancer, liver cancer, colorectal cancer, bladder cancer, breast cancer, prostate cancer, lymphatic hematopoietic tumors, glioma, soft tissue sarcoma and other malignant tumors.

Tips: cooperate with the doctor's examination. Normal value

negative.

Clinical significance

(1) Hepatocellular carcinoma patients have a certain proportion of p53 protein overexpression, and p53 gene mutation may be related to the degree of malignant differentiation of liver cancer.

(2) p53 mutations can also be found in gastric cancer, liver cancer, colorectal cancer, bladder cancer, breast cancer, prostate cancer, lymphatic hematopoietic tumors, glioblastoma, soft tissue sarcoma and other malignant tumors.

Precautions

Using sequencing or p53 gene function analysis to determine whether p53 mutations can be used for early diagnosis of various tumors.

Inspection process

Principle of determination of p53 protein: The excited product produced by many organic compounds in a chemical reaction, the light emitted when returning to the ground state is called chemi-luminescence (CL).

Reagents:

(1) Chemiluminescent compounds such as isoluminol and its derivatives.

(2) Chemiluminescent oxidation systems, such as hydrogen peroxide-peroxidase.

Not suitable for the crowd

no.

Adverse reactions and risks

no.