Refers to the detection of protozoal antigens in the blood of E. histolytica in the human intestinal tract. Normal values: enzyme-linked immunosorbent assay, indirect immunofluorescence, direct immunofluorescence: negative.

Basic Information

Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection

Applicable gender: whether men and women apply fasting: not fasting

Analysis results:

Below normal:

Normal value:
no

Above normal:

negative:
normal.

Positive:
Positive in amebic liver abscess, intestinal amebiasis or worms.

Reminder: Do not collect too much feces to avoid having enough specimens for inspection. Normal value

Enzyme-linked immunosorbent assay, indirect immunofluorescence, and direct immunofluorescence were negative.

Clinical significance

The most reliable diagnosis of amebiasis is based on direct smear microscopy of the amoeba in the lysate (trophozoites or cysts), but misdiagnosis and missed diagnosis. Immunization test can make up for the deficiency of fecal microscopy.

The clinical manifestations of amebiasis can vary greatly depending on the parasitic parts of the worm and the functional status of the host. The carrier (the worm body only colonizes the human intestine) does not have any clinical manifestations. When the worm invades the intestinal wall of the host and other tissues, it can produce a variety of clinical symptoms. The most common clinical types are amoebic dysentery and amoebic liver abscess.

Positive in amebic liver abscess, intestinal amebiasis or worms.

Precautions

1. Please avoid digging the part of the toilet urine and tap water when collecting; do not place the feces directly on toilet paper or paper towel.

2. To avoid interference with the test results, do not use cotton swabs to dig.

3. Do not collect too much feces to avoid having enough specimens for inspection.

4. If you need to detect fecal occult blood by chemical method, please avoid eating red meat, liver and spinach, cabbage, broccoli and other foods three days before collection to avoid false positive results.

5. If you use the immunological method to detect fecal occult blood, you do not need to limit the type of diet.

6. Because infants and young children are not easy to get enough samples at one time, if they need to be collected separately, please temporarily store the samples in the refrigerator to avoid bacterial growth.

Inspection process

The matrix tablets of the antigen matrix sheets (such as various tissue frozen sections or cultured cells, etc.) were taken out from the refrigerator and immediately air-dried. Before use, it is treated with anhydrous ethanol or a fixing agent such as acetone or methanol (the fixing agent is selected according to the experimental requirements). Generally fixed for 3 to 15 minutes, dry at room temperature. The test substance (serum, cerebrospinal fluid or other exudate, etc.) is appropriately diluted (1:5 or 1:10) with PBS onto the antigen matrix sheet as needed, and allowed to react at room temperature or 37 ° C in an incubator for 30 min to 1 h. Rinse with 0.01 mol/L pH 7.2 PBS for 15 min (replace PBS 3 times). Fluorescently labeled antibody was placed at room temperature or 37 ° C incubator for 30 min to 1 h. Rinse with PBS (3), buffer glycerol (pH 7.2) and slide with fluorescence microscopy.

Not suitable for the crowd

There are no taboos.

Adverse reactions and risks

There are no related complications and hazards.