Leukocyte migration inhibition test
Sensitized lymphocytes are stimulated by specific antigens in vitro and release a variety of lymphokines, including mobile inhibitors. Reflects organ and tissue transplant rejection. The mobile index is in the normal range, indicating that the receptor is in a stable phase. >1.20 indicates that the receptor is sensitive to the transplanted organ or tissue antigen.Basic Information
Specialist classification: growth and development check classification: immunological examination
Applicable gender: whether men and women apply fasting: not fastingReminder: In order to prevent contamination from affecting the results, all equipment requires sterility, and the operation process is required to be carried out under aseptic conditions. Normal value
The movement index is 1.0 ± 0.3.Clinical significance
Reflects organ and tissue transplant rejection. The mobile index is in the normal range, indicating that the receptor is in a stable phase. >1.20 indicates that the receptor is sensitive to the transplanted organ or tissue antigen.Precautions
(1) Capillary inner diameter requirements are consistent.
(2) When perfusing cells in a capillary, the cell fluid should be thoroughly mixed to avoid affecting the results due to differences in the concentration of each tube.Inspection process
(1) Heparin anticoagulation 3 to 5 ml, leukocytes were separated by stratified liquid, washed twice with Hank's solution, and counted and made into 8×107 cells/ml with cell culture solution.
(2) The white blood cell suspension is dispensed into a capillary tube in the same manner as before. 8 to 10 capillaries can be dispensed per 3 ml of blood. The lymphocyte fluids charged into each capillary are required to be equal. The end of the capillary was closed with a flame, and centrifuged in a horizontal centrifuge for 1000 r/min for 10 min. After centrifugation, the cells were compressed to a height of 4 to 6 mm, the surface layer was mainly from white blood cells, and the lower layer was reddened to red and white cells. Saw the capillary at the junction of the cell and the liquid. Use a small amount of petrolatum to adhere the closed end to the bottom of the culture chamber. Each chamber can hold 1 or 2 capillaries.
(3) Each 3 to 4 capillaries are divided into one group, one of which is filled with a culture solution, and the other or groups of cells are filled with a culture solution containing a certain concentration of antigen (be careful not to make the capillary and the culture chamber Leave bubbles). The chamber was closed with a cover glass and incubated at 37 ° C for 24 h. The white blood cell moving area in the capillary was measured as in the above method. The test results were expressed by the mobile index (MI).Not suitable for the crowd
There are no taboos.Adverse reactions and risks
There are no related complications and hazards.