Unheated serum serotonin experiment

The unheated serotonin assay uses a modified VDRL antigen, which is diluted with a dilution of the VDRL antigen and then pelleted by centrifugation. EDTA, choline chloride and a preservative are added to the precipitate, and the sensitivity and specificity are similar to those of VDRL. EDTA can make the antigen invariant within half a year, choline chloride can inactivate complement, etc., and serum can be inactivated without heat. The antigen does not have to be prepared every day and can be stored for half a year in a 4-8 °C refrigerator. The cardiolipin is used as an antigen to measure anti-cardiolipin antibodies in serum, also known as responsive factors. The test is highly sensitive and low in specificity, and is prone to biological false positives. After adequate treatment of patients with early syphilis, the responsiveness can disappear, and the early untreated patients can be reduced or disappeared in some patients. Currently, screening and quantitative tests are generally used to observe efficacy, recurrence and reinfection.

Basic Information

Specialist classification: growth and development check classification: immunological examination

Applicable gender: whether men and women apply fasting: not fasting

Analysis results:

Below normal:

Normal value:

Above normal:

Negative normal.

A positive indication may indicate a syphilis infection.

Tips: Prohibit unclean sexual intercourse. Normal value

The reaction was negative.

Clinical significance

One of the commonly used inspection methods in syphilis laboratories. Non-treponema pallidum antigen test:

Abnormal results syphilis has different abnormal results in different periods.

1 stage syphilis. That is, hard chancre, with an incubation period of 2 to 4 weeks, a dark red hard mass, a shallow ulcer, a cartilage-like hardness, and a swollen peripheral lymph node.

2 second phase syphilis. After 1 to 2 months of primary syphilis, sympathetic rash, rash, pimples, pustular rash, etc. occur in the skin and mucous membranes of the whole body. Mucosal plaques, flat wet phlegm can occur in the mucosa, and the infection is strong.

3 third-phase syphilis. Occurred 2 to 3 years or even 10 years after infection, the skin is a gum-like swelling, and may also involve bone, joint, heart, blood vessels, manifesting aortic inflammation, aortic insufficiency and aortic aneurysm, etc., invading the nerve Spinal cord paralysis, generalized paralysis (paralytic dementia). Congenital syphilis has early congenital syphilis, equivalent to the second day of the day, but heavier. Late congenital syphilis is similar to the acquired day, but few heart, blood vessel and neuropathy occur. Mainly for substantial keratitis, neurological deafness, Hutchinson's teeth (central incisors in the upper incisors, small upper and upper, equal width and width), sabre-shaped humerus and so on. There may be latent syphilis between stages, asymptomatic, and only seropositive. The treatment uses penicillin or erythromycin, tetracycline, and the like.

The person in need of examination has the above symptoms.


Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene.

Requirements for inspection: Actively cooperate with the doctor.

Inspection process

1. Qualitative experiment of slide:

(1) Aspirate 0.05 ml of the serum to be tested (not necessarily inactivated), add it to the circle of the slide, and disperse into the entire circle. The antigen was aspirated with a special syringe of 1 ml syringe, and 1 drop was added to each specimen to be examined, and the slide was shaken for 4 minutes to observe the result.

(2) Judgment of results: First observe with the naked eye, and then observe the antigen particles or agglutination precipitate with a low magnification microscope (magnification 100 times), and record the experimental results as follows.

-: The particles are fine and evenly distributed.

+-: The particles are irregularly distributed or are finely rough.

+: Small pieces are visible under the microscope and evenly distributed.

++: Small pieces are visible to the naked eye, and larger pieces are visible under the microscope, and the suspension is clear.

+++∽++++: Large or large blocks are visible to the naked eye.

Semi-quantitative experiments on slide semi-quantitative experimental slides [(++) and above] were semi-quantitative experiments for further diagnosis. First, the serum to be tested is diluted with 0.9% sodium chloride into 6 dilutions, that is, serum stocks 1:2, 1:4, 1:8, 1:16, 1:32, and 0.05 ml of diluted serum is added to the glass. Within the circle of the slice, the results are determined and tested according to qualitative methods.

Not suitable for the crowd

Inappropriate crowd: temporarily unknown.

Adverse reactions and risks

There are no related complications and hazards.