Dinitrochlorobenzene (DNCB) is a chemical that is a hapten that binds covalently to the soluble portion of the epithelin to become a complete antigen. DNCB is a pale yellow crystal grain and is soluble in acetone. 0.1 ml (containing 1-2 mg of DNCB) was dropped on the inner surface of the inner arm of the upper arm of a 2 cm diameter ring, and uniformly coated. After the acetone was volatilized, the ring was removed. After 14 days, 0.1 ml (containing 50 μg or 100 μg DNCB) was reapplied to the original sensitized skin.

Basic Information

Specialist classification: Oncology examination classification: immune examination

Applicable gender: whether men and women apply fasting: not fasting

Analysis results:

Below normal:

Normal value:
no

Above normal:

negative:
If it is negative, it may be a cellular immune defect. Tumor-type leprosy and Sjogren's syndrome are often negative.

Positive:
The positive test indicates that the cellular immune function of the body is normal. It is often used to estimate the prognosis of surgical resection of malignant tumors. In patients with positive preoperative skin test, the incidence of metastasis within 6 months after surgery is lower than that of preoperative skin test.

Tips: Actively cooperate with the doctor during the examination. Normal value

Positive.

Clinical significance

1 If it is negative, it may be a cellular immune defect. Tumor-type leprosy and Sjogren's syndrome are often negative.

2 The positive test indicates that the cellular immune function of the body is normal. It is often used to estimate the prognosis of surgical resection of malignant tumors. In patients with positive preoperative skin test, the incidence of metastasis within 6 months after surgery is lower than that of preoperative skin test.

Precautions

In the immunoassay, the microplate reader is the most commonly used instrument, and the calibration of the instrument must be carried out regularly (usually used for less than half a year, generally not more than one year).

Calibration should be performed by the manufacturer to calibrate the performance light path and give parameters when the instrument is purchased. At this time, the function calibration should be done, and the fixed value calibrator (including different titers and yin and yang specimens) should be selected for 4-5 days. The sampler and the water bath thermometer which have great influence on the experiment should be used after measurement and calibration. The former can be calibrated by weighing method, and the latter can be carried out by the national measurement unit.

Inspection process

The lavender produced by the interaction of dinitrochlorobenzene with a strong base (turned orange after standing) was subjected to colorimetric determination.

Not suitable for the crowd

There are no taboos.

Adverse reactions and risks

There are no related complications and hazards.