Congo red skin experiment
Congo red intradermal test is a diagnosis of multiple myeloma; chronic infection; macroglobulinemia; nephrotic syndrome, skin amyloidosis.Basic Information
Specialist classification: cardiovascular examination classification: immune examination
Applicable gender: whether men and women apply fasting: not fastingAnalysis results:
Urine was negative; plasma (Congo red 60 min retention) > 0.06 (> 60%).
Normal people are negative. Positive, immediate response and delayed response. The immediate reaction usually takes place within 5 to 30 minutes, and if a wheal is produced, it is positive.Clinical significance
When the serum retention rate is <40% and there is no Congo red in the urine, it is mainly seen in renal amyloidosis, and also in multiple myeloma, long-term chronic infectious diseases and macroglobulinemia. Nephrotic syndrome has a false retention rate, because the protein in the urine can be adsorbed together with the non-toxic dye, and the urine is characterized by red.
Abnormal results renal amyloidosis, also seen in multiple myeloma, long-term chronic infectious diseases and macroglobulinemia.
People who need to check:
Multiple myeloma; chronic infection; macroglobulinemia; nephrotic syndrome, skin amyloidosis in patients.Precautions
Contraindications before examination: Small-dose skin tests for people with skin allergies.
Requirements for examination: slow reaction, usually only after a few hours to 48 hours, such as invasive nodules, which is positive.Inspection process
Laboratory examination: there may be accelerated erythrocyte sedimentation rate, abnormal globulin, elevated gamma or alpha globulin. In the Nomland test, a 1.5% Congo red solution was injected into a suspected rash (intradermal). After 24 to 48 hours, only red was left in the amyloid protein, and the positive rate was 80% by skin microscopy.
The left upper limb or the right upper limb of the patient is exposed by the operator, and the prepared, sequentially specific antigen is sequentially injected intradermally after the skin is disinfected and treated on the back. After 15 minutes, the skin reaction of the test area was observed, and the degree of reaction was judged according to the degree of the reaction, so that the treatment plan and means were determined according to the degree of the disease.Not suitable for the crowd
Inappropriate crowd: People without any of the above symptoms.Adverse reactions and risks
There are no related complications and hazards.