Anti-thyroxine receptor antibody (TRAb)
Thyrotropin receptor antibody (TRAb) is also known as thyroid stimulating antibody (TSAb) or thyroid stimulating immunoglobulin (TSI). TRAb is a thyroid autoantibody. It is also divided into thyroid stimulating antibody (TSAb) and thyroid blocking antibody (TBAb) according to physiological effects. It is produced during the autoimmune process of toxic diffuse goiter and can stimulate the thyroid gland. The production of thyroid hormone, the determination of TRAb is conducive to the study of the pathogenesis of diffuse toxic goiter. Anti-thyroxine receptor antibody assays were determined by ELISA. The ELISA method is a highly sensitive test technique that combines the specific reaction of antigens and antibodies with the efficient catalytic action of enzymes on substrates based on immunological reactions. The reaction of the antigen and the antibody is carried out in a well of a solid phase carrier, a polystyrene microtiter plate, and after each reagent is added, the excess free reactant can be removed by washing to ensure the specificity of the test result. stability.Basic Information
Specialist classification: growth and development check classification: immunological examination
Applicable gender: whether men and women apply fasting: fastingAnalysis results:
Normal ELISA: 10%.
A positive indication may be hyperthyroidism or thyroiditis with hypothyroidism.
ELISA <10%.Clinical significance
TSAb positive can be seen in autoimmune hyperthyroidism, neonatal transient hyperthyroidism.
TBAb-positive thyroiditis with hypothyroidism, idiopathic hypothyroidism.
Note that when both are present, the thyroid function depends on the relative strength of the two.Precautions
First, the precautions before blood draw:
1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results.
2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results.
3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection.
Second, after blood draw should pay attention to:
1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma.
2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended.
3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved.
4. If there is localized congestion, use a warm towel after 24 hours to promote absorption.
3. Please inform the doctor about the recent medication and special physiological changes before the test.Inspection process
The known unlabeled specific antibody (primary antibody) is reacted with the antigen sample, the unreacted antibody is washed away with water, and the labeled anti-antibody (second antibody) is reacted with the antigen sample to form an antigen-antibody- The antibody complex is washed away with unreacted labeled antibody, dried, sealed and microscopically examined.Not suitable for the crowd
There are no taboos.Adverse reactions and risks
There are no related complications and hazards.