The anti-hepatocyte membrane antigen antibody (LMA) was originally discovered by Hopf et al. in the serum of HBsAg-negative chronic active hepatitis and cirrhosis patients. The target antigen is a liver cell membrane soluble protein different from LSP, which can be centrifuged from liver homogenate. After separation and purification in the supernatant, it also has hepatocyte specificity without strict species specificity. In immunoelectrophoresis, LM-Ag is in the prealbumin zone (while LSP is in the gamma zone). The presence of LMA autoantibodies is an important factor in the analysis of hepatocyte injury following anti-LSP discovery, which is said to be mediated by antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-mediated cytotoxicity (CDC). Causes damage to liver cells.

Basic Information

Specialist classification: Digestive examination classification: immune examination

Applicable gender: whether men and women apply fasting: not fasting

Analysis results:

Below normal:

Normal value:
no

Above normal:

negative:
normal.

Positive:
Prompt for the presence of hepatitis.

Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. Normal value

Normal human serum LMA is negative.

Clinical significance

LMA is of great value in the identification of autoimmune chronic active hepatitis (also known as lupus hepatitis) and slow-lived liver caused by hepatitis B virus. LMA has a higher frequency of detection in patients with chronic immune-induced liver disease (CALD) and primary biliary cirrhosis with autoimmune and unexplained, and the positive rate can reach 83%, 47% and 42%, respectively. In patients with chronic liver disease who were HBsAg-positive or HBsAg-negative but anti-HBc-positive, the LMA-positive rate was 11%.

Precautions

First, the precautions before blood draw:

1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results.

2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results.

3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection.

Second, after blood draw should pay attention to:

1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma.

2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended.

3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved.

4. If there is localized congestion, use a warm towel after 24 hours to promote absorption.

3. Please inform the doctor about the recent medication and special physiological changes before the test.

Inspection process

Same as indirect fluorescence method.

Not suitable for the crowd

There are no taboos.

Adverse reactions and risks

There are no related complications and hazards.