A method of determining the infection of measles virus. Positive for measles virus infection. The IgM antibody of measles virus can appear early in the onset, peaks in about 10 days, and disappears after 30 to 60 days. The IgG antibody and the lgM antibody appeared at the same time or later, and decreased significantly within 6 months, and then maintained at a certain level and eventually survived.

Basic Information

Specialist classification: Infectious disease examination and classification: immunological examination

Applicable gender: whether men and women apply fasting: not fasting

Analysis results:

Below normal:

Normal value:
no

Above normal:

negative:
normal.

Positive:
Abnormally suggest measles virus infection.

Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. Normal value

Measles virus antibody IgM is negative.

Clinical significance

Positive measles virus infection.

Precautions

First, the precautions before blood draw:

1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results.

2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results.

3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection.

Second, after blood draw should pay attention to:

1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma.

2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended.

3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved.

4. If there is localized congestion, use a warm towel after 24 hours to promote absorption.

3. Please inform the doctor about the recent medication and special physiological changes before the test.

Inspection process

The measles antigen and the control antigen were separately diluted with a carbonate buffer, and ELISA plates were individually coated in parallel with 100 μl per well. 4 ° C overnight. The serum to be tested was diluted to 1:100 with a dilution solution, 100 μl of each of the measles antigen well and the control antigen well was added, and the anti-human IgG enzyme conjugate was diluted with a sample dilution solution at a temperature of 37 ° C for 1 to 2 hours, 100 μl per well. At 37 ° C for 1 ~ 2h, add substrate 100μl / well. After 10 to 15 min at 37 ° C, the reaction was terminated with 2 mol/L H 2 SO 450 μl/well.

Not suitable for the crowd

There are no taboos.

Adverse reactions and risks

There are no related complications and hazards.