The internal factor is a glycoprotein that binds to vitamin B12 to form a complex that binds to the receptor in the ileal mucosa and is absorbed. Anti-internal factor (AIFA) is a type II blocking antibody that inhibits the binding of endogenous factors to vitamin B12; type II is a binding antibody that binds to the inner factor-vitamin B12 complex and blocks the complex and ileal mucosal receptors. Attached. Anti-internal factor antibodies are commonly determined by ELISA. ELISA is a highly sensitive test technique that combines the specific reaction of antigens and antibodies with the efficient catalysis of enzymes on substrates based on immunological reactions. The reaction of the antigen and the antibody is carried out in a well of a solid phase carrier, a polystyrene microtiter plate, and after each reagent is added, the excess free reactant can be removed by washing to ensure the specificity of the test result. stability.

Basic Information

Specialist classification: growth and development check classification: immunological examination

Applicable gender: whether men and women apply fasting: not fasting

Analysis results:

Below normal:

Normal value:
no

Above normal:

negative:
normal.

Positive:
Prompt for pernicious anemia.

Reminder: The reaction of antigen and antibody is carried out in the well of a solid phase carrier, polystyrene microtiter plate. After each reagent is added, the excess free reactant can be removed by washing to ensure the test result. Specificity and stability. Normal value

Negative ELISA.

Clinical significance

Positive:

Type I AIFA is a specific autoantibody for pernicious anemia, which is positive in the early stage of pernicious anemia; type II occurs in the terminal stage of hypertrophy of gastric mucosa in malignant anemia.

AiFA-positive people with pernicious anemia usually have positive parietal cell antibodies, and AIFA-positive parietal cell-negative patients are rare.

Precautions

Anti-internal factor antibodies are commonly determined by ELISA. ELISA is a highly sensitive test technique that combines the specific reaction of antigens and antibodies with the efficient catalysis of enzymes on substrates based on immunological reactions. The reaction of the antigen and the antibody is carried out in a well of a solid phase carrier, a polystyrene microtiter plate, and after each reagent is added, the excess free reactant can be removed by washing to ensure the specificity of the test result. stability.

Inspection process

Determined by the RIA method.

Not suitable for the crowd

There are no taboos.

Adverse reactions and risks

There are no related complications and hazards.