Anti-parietal cell antibody
Anti-parietal cell antibody (PCA) was first discovered in the serum of patients with pernicious anemia by Taylor et al. The target antigen is localized in the membrane of the parietal microvilli, which is a microsome part of the cytoplasm and a cytoplasmic membrane. lipoprotein. PCA is organ-specific and does not react with other organs other than the stomach, but has no species specificity. Therefore, rat or rabbit gastric parietal cells can be used as antigens. The Ig category of PCA is mainly IgG and IgA (also a small amount of IgM), IgG is the main serum, and IgA is more common in gastric juice.Basic Information
Specialist classification: Digestive examination classification: blood examination
Applicable gender: whether men and women apply fasting: fastingAnalysis results:
Patients with pernicious anemia (PA) with atrophic gastritis 80%-100% PCA-positive, atrophic gastritis without malignant anemia 40%-60% positive, hyperthyroidism, lymphocytic thyroiditis, diabetes, iron deficiency anemia Positive.
(1) Anti-gastric cell antibody (PCA) 2% to 10%.
(2) Anti-intragastric factor antibody (IFA).
Type 1 antibody 0%.
2 type II antibody 0%.Clinical significance
Abnormal results: 80% to 100% PCA-positive, atrophic gastritis with atrophic gastritis combined with atrophic gastritis, 40% to 60% positive for atrophic gastritis, hyperthyroidism, lymphocytic thyroiditis, diabetes, iron deficiency Anemia is positive. In addition, 50% to 75% of PA patients can be seen with IFAI type antibodies and type II antibodies 30% to 50% positive.Positive results may be diseases: gastritis, gastric ulcer precautions
Pre-examination contraindications: Take a large number of drugs that affect antibodies against gastric parietal cells.
Requirements for examination: 2ml of venous blood, no anticoagulation, separation of serum for determination. This test has nothing to do with the positive gender. During the examination, the patient pays attention to the doctor and waits patiently.Inspection process
The conventional detection method is an IIF method using a mouse stomach frozen section as an antigen matrix. The fluorescent pattern is dyed in the fine granules of the cytoplasm of the parietal cells, and is often strongly stained at the apex. The mouse stomach is a suitable substrate because a false positive reaction occurs in the stomach of the rat. An ELISA established by antigen purification by affinity chromatography is also a preferred method.Not suitable for the crowd
Inappropriate crowd: no special population.Adverse reactions and risks
No special complications.