Anti-small intestine goblet cell antibodies can help diagnose inflammatory bowel disease, which is usually determined by indirect immunofluorescence. High titer anti-small goblet cell antibodies are mainly found in ulcerative colitis, with a positive rate of 28% to 39%. Some Crohn's disease patients can also detect antibodies against small intestinal goblet cells. Anti-small intestinal goblet cell antibodies were detected in close relatives of patients with inflammatory bowel disease, indicating prone to inflammatory bowel disease.

Basic Information

Specialist classification: Digestive examination classification: blood examination

Applicable gender: whether men and women apply fasting: fasting

Analysis results:

Below normal:

Normal value:
no

Above normal:

negative:
Normal: Indirect immunofluorescence: negative.

Positive:
Positive: High titer anti-small goblet cell antibodies are mainly found in ulcerative colitis, with a positive rate of 28% to 39%. Some Crohn's disease patients can also detect antibodies against small intestinal goblet cells. Anti-small intestinal goblet cell antibodies were detected in close relatives of patients with inflammatory bowel disease, indicating prone to inflammatory bowel disease.

Tips: After 8 pm on the day before the medical examination, you should start fasting for 12 hours, so as not to affect the test results. Normal value

Indirect immunofluorescence was negative.

Clinical significance

Positive:

High titer anti-small goblet cell antibodies are mainly found in ulcerative colitis, with a positive rate of 28% to 39%. Some Crohn's disease patients can also detect antibodies against small intestinal goblet cells. Anti-small intestinal goblet cell antibodies were detected in close relatives of patients with inflammatory bowel disease, indicating prone to inflammatory bowel disease.

Need to check patients with colitis in the population.

Precautions

Forbidden before examination: Please inform the doctor about the recent medication and special physiological changes before the test.

1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results.

2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results.

3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection.

After inspection:

1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma.

2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended.

3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved.

4. If there is localized congestion, use a warm towel after 24 hours to promote absorption.

Inspection process

Indirect immunofluorescence experimental principle: fluorescein is labeled on the corresponding antibody and directly reacts with the corresponding antigen.

In the first step, an unknown unlabeled antibody (sample to be tested) is added to a known antigen sample, and incubated at 37 ° C for 30 min in a wet box to sufficiently bind the antigen antibody, followed by washing to remove unbound antibody.

In the second step, a fluorescently labeled anti-globulin antibody or an anti-IgG, IgM antibody is added. If an antigen-antibody reaction occurs in the first step, the labeled [de]antiglobulin antibody will further bind to the antigen-binding antibody, thereby identifying an unknown antibody.

Not suitable for the crowd

Inappropriate crowd: no special population.

Adverse reactions and risks

There are no related complications.