Diabetic patients receiving heterologous pancreatic hormone therapy often have anti-insulin antibodies (IAB) in their serum. This antibody binds to insulin to form a complex that inactivates insulin, which is the main cause of insulin resistance in diabetic patients. One. It has been confirmed that five kinds of Ig-type anti-insulin antibodies exist, but mainly IgGs, and there are various methods for measuring the antibodies, such as RIA, ELISA, hemagglutination and microcrystalline cellulose.

Basic Information

Specialist classification: Digestive examination classification: endocrine examination

Applicable gender: whether men and women apply fasting: fasting

Analysis results:

Below normal:

Normal value:
no

Above normal:

negative:
normal.

Positive:
Insulin dependent diabetes.

Tips: Individual antithyroid antibodies can also be detected in patients with hyperthyroidism. Normal value

Normal human serum anti-insulin antibodies are negative.

Clinical significance

Abnormal results: serum anti-insulin antibodies are positive:

(1) Guiding insulin-dependent diabetes patients in the treatment cycle, high titer of insulin antibodies is the main cause of severe insulin resistance. A-IAb can guide the amount of insulin to provide treatment for drug-resistant diabetes. Long-acting insulin is used when moderately increasing the titer of fast-acting insulin antibodies.

(2) Determining the prognosis of insulin-dependent diabetes The insulin release curve is low and the high insulin antibody titer indicates that the patient is not islet failure indicating that the disease is stable, indicating that the prognosis of insulin failure is poor.

(3) Anti-insulin antibodies can also be detected in patients with hyperthyroidism.

Patients with population-dependent diabetes mellitus and hyperthyroidism need to be examined.

Positive results may be diseases: multi-gland deficiency syndrome considerations

Pre-examination contraindications: Anti-insulin antibodies are produced by injection of exogenous insulin, and even intermittent use can often cause reminiscent antibody reactions. Patients should draw attention in this regard.

Requirements for inspection:

(1) Judging the prognosis of insulin-dependent diabetes: the insulin release curve is low and the insulin antibody titer is high, indicating that the patient is not islet failure, suggesting that the condition is stable. On the contrary, it indicates insulin failure and has a poor prognosis.

(2) Anti-insulin antibodies can also be detected in patients with hyperthyroidism.

(3) When the serum to be tested contains anti-insulin antibodies at the same time, the results of the anti-insulin receptor antibody (IRA) are interfered with and need to be corrected by other methods.

Inspection process

How to detect anti-insulin antibodies, insulin-dependent diabetes is caused by a lack of insulin secretion caused by metabolic disorders, requiring a large amount of exogenous insulin to maintain life. Anti-insulin antibodies are produced by the injection of exogenous insulin, and can often cause reminiscent antibody reactions even when used intermittently. The method for detecting insulin antibodies includes an ELISA method, an immunospot method, and the like, and an ELISA method is now described.

We can use purified human, pig or bovine crystalline insulin for coating, the test serum is diluted 1:10, plus the optimal amount of working concentration of HRP-labeled anti-human IgC antibody or labeled anti-human IgA insulin antibody, plus substrate Color development, positive results were followed by the highest dilution of serum insulin antibody b.

Not suitable for the crowd

Inappropriate people: generally no special population.

Adverse reactions and risks

No related complications or hazards.