Anti-islet cell antibody

Anti-islet cell antibody (ICA) was first reported by Bottazzo in 1974. The target antigen is a component present in the cytoplasm or microsomes of islet cells, organ-specific, but species-specific, human islet cells The antibodies cross-reacted with islet cells of monkey, rat and guinea pigs. Normal people do not have this antibody. The positive rate of 40% to 50% in patients with diabetes onset is 15% to 20% in 5-10 years, especially in juvenile diabetes. This antibody primarily distinguishes between insulin-dependent diabetes mellitus (1DDM) and non-insulin-dependent diabetes mellitus (NIDDM). In the new ID-DM patients, the positive rate can reach more than 90%, and ICA positive in NIDDM patients can predict the possibility of IDDM.

Basic Information

Specialist classification: Digestive examination classification: endocrine examination

Applicable gender: whether men and women apply fasting: fasting

Analysis results:

Below normal:

Normal value:

Above normal:


Diabetic patients.

Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. Normal value


Clinical significance

Positive in the initial diagnosis of insulin-dependent diabetes mellitus, the total positive rate was 31.2, up to 85% in the first two weeks of onset, and reduced to 50% in a few weeks; in non-insulin-dependent diabetes and "chemical" diabetes Humans, the positive rate is only 6.2% and 4.9%, so it can be used as a differential diagnosis basis for insulin-dependent and independent diabetes.

Positive results may be diseases: considerations for diabetes

Forbidden before examination: Please inform the doctor about the recent medication and special physiological changes before the test.

1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results.

2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results.

Requirements for examination: When taking blood, you should relax your mind, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection. Obtain serum samples and store at -20 degrees Celsius.

Inspection process

Indirect immunofluorescence experimental principle: fluorescein is labeled on the corresponding antibody and directly reacts with the corresponding antigen.

In the first step, an unknown unlabeled antibody (sample to be tested) is added to a known antigen sample, and incubated at 37 ° C for 30 min in a wet box to sufficiently bind the antigen antibody, followed by washing to remove unbound antibody.

In the second step, a fluorescently labeled anti-globulin antibody or an anti-IgG, IgM antibody is added. If an antigen-antibody reaction occurs in the first step, the labeled anti-globulin antibody is further bound to the antigen-binding antibody, thereby enabling identification. Unknown antibody.

Not suitable for the crowd

Inappropriate people: generally no special population.

Adverse reactions and risks

There are no related complications and hazards.