Centromere, also known as centromere, is a narrow segmental structure in the chromosome. Before cell division, each chromosome consists of two chromatids with identical genes, which are joined together at the centromere, and in mitosis, respectively, connected to the traction wires of the spindle poles, staining the two The monomers are pulled in the direction of their respective centrosomes. The centromere antigen consists of three centromeric proteins (CenP), namely CenP-A (17 kD), CenP-B (80 kD), and CenP-C (140 kD). CenP-A is a centromere-specific core histone that may play a direct role in the packaging and function of chromosome centromeres. CenP-B is a major component of the centromere antigen and is a DNA-binding protein rich in alpha satellite DNA (also known as satellite DNA), which is concentrated in the dividing chromosome and the nucleus of the interphase. CenP-C is a large molecular weight protein in centromere antigens, and its role is unknown. In dividing cells, the centromere antigen is separated from the concentrated chromosome, and in the interphase of cell division, the antigen is present on the concentrated chromosome, and on the stretched single chromosome, the antigen is located in the main scar region of the chromosome. Anti-centromere antibodies are a limitation in systemic clerosis (CREST), such as CREST [calcinosls calcium deposition, Raynaud'sphenomenon Raynaud's phenomenon, esophagealdysmotility esophageal dyskinesia, and sclerodactyly (toe) scleroderma. The telangiectasis telangiectasia syndrome marker antibody, there are many reports that this antibody and anti-Scl-70 antibody mutually exclusive, both positive patients are extremely rare.

Basic Information

Specialist classification: skin examination classification: blood examination

Applicable gender: whether men and women apply fasting: fasting

Analysis results:

Below normal:

Normal value:
no

Above normal:

negative:
Normal humans are negative for anti-centromere antibodies.

Positive:
Positive: anti-centromere antibody.

Tips: Try to follow the doctor's instructions to do the relevant checks. Normal value

Normal humans are negative for anti-centromere antibodies.

Clinical significance

In patients with CREST syndrome, the anti-centromere antibody detection rate is 80% to 90%, which has a diagnostic significance for the syndrome. In addition, anti-centromere antibodies can also be positive in approximately 25% of patients with primary Raynaud's phenomenon (no other symptoms or signs of CREST syndrome). These patients may be early variants or frustrations of CREST syndrome because some of them develop into a typical CREST syndrome in a few years. CREST patients who are anti-centrosome-positive are less likely to have skin and visceral involvement than antibody-negative ones. In patients with systemic progressive sclerosis (PSS), the positive rate of anti-centromere antibodies is 22% to 36%. In addition, there are some patients with SLE, Sjogren's syndrome, RA or Hashimoto's thyroiditis with Raynaud's phenomenon. Anti-centromere antibodies were detected.

Positive results may be diseases: pediatric scleroderma, eosinophilia-myalgia syndrome, Raynaud's syndrome, Raynaud's disease considerations

Inappropriate people: generally no special population.

Taboo before inspection: There are not too many taboo points, and you can prepare yourself before the examination. You can ask the doctor how to cooperate.

Requirements for inspection: Try to follow the doctor's instructions to do the relevant inspections.

Inspection process

Establish a simple, convenient, accurate and specific method for the detection of antinuclear antibodies (ANA) and anti-centromere antibodies (ACA). Methods: HRP-SPA immunohistochemistry and indirect immunofluorescence were compared. RESULTS: The sera of patients were tested for ANA and ACA. The positive rates of ANA were basically the same in both methods, and the karyotypes were slightly different. The HRP-SPA immunohistochemical method was used to detect the ANA normal value titer was ≤1:80, and the indirect immunofluorescence normal value titer was set to ≤1:40. The positive rate of ARP by HRP-SPA immunohistochemistry was higher than that of indirect immunofluorescence, and the normal titer was the same as ANA. Conclusion: HRP-SPA immunohistochemistry can completely replace indirect immunofluorescence for ANA and ACA detection. It is a simple and rapid test technique for screening primary rheumatism in primary medical units.

Not suitable for the crowd

There are no special taboos.

Adverse reactions and risks

There are no related complications and hazards.